Zgleszewski S E, Blewett C J, Cilley R E, Krummel T M, Chinoy M R
Department of Surgery, The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey 17033, USA.
J Pediatr Surg. 1998 Mar;33(3):433-41. doi: 10.1016/s0022-3468(98)90084-7.
The authors hypothesized that increased intraluminal pressure in the fetal stomach would enhance development in a murine organ culture model.
Gestation day 14 (Gd14) fetal stomachs from time-dated pregnant CD-1 mice (term, 20 days) were maintained in organ culture for 7 days. Some stomachs were ligated at the gastroesophageal (GE) and pyloroduodenal (PD) junctions. Others were left unligated. Gd14, Gd16, and Gd18 stomachs were taken as well to compare organogenesis in vivo. Tissues were processed for histological, morphometric, and immunohistochemical analysis, as well as total protein and DNA determination.
The ligated stomachs were visibly distended compared with unligated stomachs in organ culture after 7 days. The length and width of the 7-day in vitro ligated stomachs were significantly increased compared with unligated (2.97+/-0.04 mm v 2.48+/-0.05 mm and 2.14+/-0.04 mm v 1.57+/-0.08 mm, respectively, P < .05). Mucosal epithelial cells showed nuclear polarization, and there was a distinct outer muscle layer in the ligated stomachs, but not in the unligated stomachs, which demonstrated pseudostratified epithelial cells in the mucosa. The ligated stomachs had increased in mucosal thickness compared with unligated (31.4+/-1.3 microm vs 24.9+/-0.9 microm, p < 0.05). The ligated stomachs also had significantly increased protein and DNA content when compared with unligated stomachs (65.8+/-3.1 microg and 23.3+/-1.2 microg v 55.0+/-2.7 microg and 19.0+/-1.2 microg, respectively, P < .05). However, there were no significant differences noted between the protein to DNA ratios. Immunohistochemical staining for proliferating cell nuclear antigen (PCNA), a marker for cell proliferation, demonstrated increased proliferative activity of the mucosal epithelial cells in the ligated stomachs.
Esophageal and pyloric ligation enhanced the development of the fetal stomach in vitro in comparison with unligated stomachs cultured under similar conditions. Developmental characteristics of the ligated stomachs paralleled that of Gd16 stomachs in vivo.
作者推测在小鼠器官培养模型中，胎儿胃内腔内压力升高会促进其发育。
选取妊娠第14天（Gd14）、来自定时受孕的CD-1小鼠（孕期20天）的胎儿胃，进行器官培养7天。部分胃在食管胃（GE）和幽门十二指肠（PD）交界处结扎，其他胃不结扎。同时选取Gd14、Gd16和Gd18的胃以比较体内器官发生情况。对组织进行组织学、形态学和免疫组织化学分析，以及总蛋白和DNA测定。
在器官培养7天后，结扎的胃与未结扎的胃相比明显扩张。体外培养7天的结扎胃的长度和宽度与未结扎胃相比显著增加（分别为2.97±0.04毫米对2.48±0.05毫米和2.14±0.04毫米对1.57±0.08毫米，P <.05）。黏膜上皮细胞显示核极化，结扎的胃中有明显的外层肌肉层，而未结扎的胃中没有，未结扎胃的黏膜显示假复层上皮细胞。与未结扎胃相比，结扎胃的黏膜厚度增加（31.4±1.3微米对24.9±0.9微米，p < 0.05）。与未结扎胃相比，结扎胃的蛋白质和DNA含量也显著增加（分别为65.8±3.1微克和23.3±1.2微克对55.0±2.7微克和19.0±1.2微克，P <.05）。然而，蛋白质与DNA的比率没有显著差异。增殖细胞核抗原（PCNA），一种细胞增殖标志物，免疫组织化学染色显示结扎胃中黏膜上皮细胞的增殖活性增加。
与在相似条件下培养的未结扎胃相比，食管和幽门结扎增强了胎儿胃在体外的发育。结扎胃的发育特征与体内Gd16胃的发育特征相似。
