Comparative study of air coagulation, fibrin sealant, and suture in experimental liver injury.

OBJECTIVE

To test the effects of hot air coagulation, fibrin sealant, and horizontal mattress sutures on haemostasis and regeneration in experimental hepatectomy.

DESIGN

Randomised laboratory experiment.

SETTING

Teaching hospital, Spain.

MATERIAL

200 rats divided into four groups (three experimental [n=60 in each] and one control [n=20]).

INTERVENTIONS

Hepatic injuries were repaired by suture, coagulation, or fibrin sealant in the three experimental groups. The control group was used only to supply baseline blood samples. 10 animals in each experimental group were killed at 3, 5, 10, 25, 40, and 60 days.

MAIN OUTCOME MEASURES

Time taken to achieve haemostasis, and histopathological scores of healing.

RESULTS

Mattress sutures took mean (SEM) of 346 (7) seconds to control the haemorrhage and allow the liver to regain its shape and 4 rats developed abscesses (7%). Fibrin sealant achieved haemostasis immediately and the liver regained its shape in 58 (2) seconds; 2 rats (3%) developed abscesses. Hot air coagulation achieved haemostasis in 27 (1) seconds and there were no abscesses.

CONCLUSION

Fibrin sealant was the best technique because it achieved immediate haemostasis and speedy regeneration. However, hot air coagulation is a useful and cheaper alternative.