Bondre V P, Sinha V B, Srivastava B S
Division of Microbiology, Central Drug Research Institute, Lucknow, India.
FEMS Immunol Med Microbiol. 1997 Dec;19(4):323-9. doi: 10.1111/j.1574-695X.1997.tb01103.x.
Various cellular fractions of Vibrio cholerae O139 were prepared and evaluated in the rabbit ileal loop model of experimental cholera for identification of the protective antigen(s) relevant for vaccine development. Lipopolysaccharides (LPS) and capsular polysaccharides (CPS) of O139 strains and its cell surface, membrane and cytosolic fractions were assayed for antibacterial immunity, whereas the cholera toxin was examined for antitoxic immunity. The lipopolysaccharides, membrane fraction and cholera toxin induced moderate protection, however there was a significant synergistic effect when cholera toxin was combined with membrane proteins or lipopolysaccharides. The O139 strains strongly resembled O1 strains in the profile of proteins and immunological cross reactivity, yet there was no cross protection. The results warrant further investigation of the pathogenesis of O139 strains and identify the critical somatic antigens relevant to protection.
制备了霍乱弧菌O139的各种细胞组分，并在实验性霍乱的兔回肠袢模型中进行评估，以鉴定与疫苗开发相关的保护性抗原。检测了O139菌株的脂多糖（LPS）、荚膜多糖（CPS）及其细胞表面、膜和胞质组分的抗菌免疫，同时检测了霍乱毒素的抗毒免疫。脂多糖、膜组分和霍乱毒素诱导了中等程度的保护作用，然而，当霍乱毒素与膜蛋白或脂多糖联合使用时，存在显著的协同效应。O139菌株在蛋白质谱和免疫交叉反应性方面与O1菌株非常相似，但不存在交叉保护作用。这些结果值得进一步研究O139菌株的发病机制，并确定与保护相关的关键菌体抗原。
