Billingsley K A, Backus S M, Juneson C, Ward O P
Microbial Biotechnology Laboratory, Department of Biology, University of Waterloo, ON, Canada.
Can J Microbiol. 1997 Dec;43(12):1172-9. doi: 10.1139/m97-166.
Resting cells of Pseudomonas strain LB400, grown on biphenyl, transformed 80, 50 and 17% of Aroclor 1242, 1254, and 1260, respectively. Resting cells grown on glucose or glycerol also transformed these polychlorinated biphenyl (PCB) mixtures to the extent of 60, 35, and 9% for Aroclors 1242, 1254, and 1260, respectively. Time courses of the transformation of the separated individual congeners in the Aroclors were plotted and used to determine the transformation rate constants (k). By analysis of the rate constants, it was concluded that the order of degradation of the different congeners in an Aroclor were similar regardless of the growth substrate. In general, k values for the conversion of a particular congener were lower for cells grown on glucose or glycerol compared with cells grown on biphenyl. Generally, k values for the transformation of the same congener in different Aroclors were not the same: rate constants had highest values for the congener in Aroclor 1242 and lowest values in Aroclor 1260. The data allowed congeners to be grouped according to their relative rates of degradation. The ratio of k values for transformation of individual congeners in Aroclors by cells grown on biphenyl and glucose were not constant.
在联苯上生长的假单胞菌LB400静止细胞分别将80%、50%和17%的Aroclor 1242、1254和1260转化。在葡萄糖或甘油上生长的静止细胞也分别将这些多氯联苯（PCB）混合物转化，Aroclor 1242、1254和1260的转化程度分别为60%、35%和9%。绘制了Aroclor中分离出的各个同系物转化的时间进程，并用于确定转化速率常数（k）。通过对速率常数的分析得出结论，无论生长底物如何，Aroclor中不同同系物的降解顺序相似。一般来说，与在联苯上生长的细胞相比，在葡萄糖或甘油上生长的细胞中特定同系物转化的k值较低。通常，不同Aroclor中相同同系物转化的k值并不相同：Aroclor 1242中同系物的速率常数最高，而在Aroclor 1260中最低。这些数据允许根据同系物的相对降解速率进行分组。在联苯和葡萄糖上生长的细胞对Aroclor中各个同系物转化的k值之比并不恒定。
