通过一种新型阳离子脂质体制剂在哺乳动物细胞中进行体外基因转移。

In vitro gene transfer in mammalian cells via a new cationic liposome formulation.

作者信息

Kao M C, Law S L, Chuang T C, Lin Y S

机构信息

Department of Biochemistry, National Defense Medical Center, P.O. Box 90048-501, Taipei, 100, Taiwan, R.O.C.

出版信息

Oncol Rep. 1998 May-Jun;5(3):625-9. doi: 10.3892/or.5.3.625.

DOI:10.3892/or.5.3.625

PMID:9538164

Abstract

A new cationic liposome formulation of sphingosine (SP) and dioleoylphosphatidylethanolamine (DOPE) was developed as an efficient transfection reagent. This SP/DOPE liposome showed efficient transfection in a wide variety of mammalian cancer cells. No significant cytotoxicity of the SP/DOPE liposome to cells was observed. The tranfection activity was greater than that of a well-reported liposome which was made from a cholesterol derivative 3beta-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and the neutral lipid DOPE. In addition, the SP/DOPE liposome was found to be less toxic to cells than the DC-Chol/DOPE liposome. Stable transfections mediated by SP/ DOPE liposome were also demonstrated. These results suggest that the SP/DOPE liposome may provide a good gene delivery system to be used in the human cancer gene therapy.

摘要

一种由鞘氨醇（SP）和二油酰磷脂酰乙醇胺（DOPE）组成的新型阳离子脂质体制剂被开发为一种高效的转染试剂。这种SP/DOPE脂质体在多种哺乳动物癌细胞中显示出高效转染能力。未观察到SP/DOPE脂质体对细胞有明显的细胞毒性。其转染活性高于一种由胆固醇衍生物3β-[N-（N'，N'-二甲基氨基乙烷）-氨基甲酰]胆固醇（DC-Chol）和中性脂质DOPE制成的、报道广泛的脂质体。此外，发现SP/DOPE脂质体对细胞的毒性比DC-Chol/DOPE脂质体小。还证明了由SP/DOPE脂质体介导的稳定转染。这些结果表明，SP/DOPE脂质体可能为人类癌症基因治疗提供一种良好的基因递送系统。