Ikawa Y, Shiraishi H, Inoue T
Department of Chemistry, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan.
J Biochem. 1998 Mar;123(3):528-33. doi: 10.1093/oxfordjournals.jbchem.a021968.
The Tetrahymena group I self-splicing intron contains peripheral domains P2-2.1. Mutant introns lacking these domains are hardly active. We found that if an independently prepared P2-2.1 RNA is added in trans, it efficiently enhances the catalytic activity of an intron lacking the domains. P2-2.1 RNA together with the previously identified activator, P5abc RNA, of the Tetrahymena intron can activate the intron lacking both of them. The trans-activation depends on the long-range interaction between P2.1 and P9.1 domains.
嗜热四膜虫I组自剪接内含子包含外周结构域P2-2.1。缺少这些结构域的突变内含子几乎没有活性。我们发现，如果以反式添加独立制备的P2-2.1 RNA，它能有效增强缺少这些结构域的内含子的催化活性。P2-2.1 RNA与先前鉴定出的嗜热四膜虫内含子激活剂P5abc RNA一起，可以激活同时缺少这两者的内含子。反式激活取决于P2.1和P9.1结构域之间的长程相互作用。
