Kudo N, Nishiyama M, Sasaki H, Abe K, Arai S, Tanokura M
Biotechnology Research Center The University of Tokyo, Bunkyo-ku, Tokyo 113-8657.
J Biochem. 1998 Apr;123(4):568-70. doi: 10.1093/oxfordjournals.jbchem.a021974.
Oryzacystatin-I from rice seeds was overexpressed in Escherichia coli, purified, and crystallized by the sitting-drop vapor diffusion method. Crystals obtained with 2-methyl-2,4-pentanediol as a precipitant exhibited space group I4122, with unit cell parameters of a = b = 100.0 A, c = 54.2 A, and diffracted up to 2.8 A resolution at 100 K. The crystals have one molecule per asymmetric unit.
