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[化学发光在人体弓形虫病血清学诊断中的应用]

[Application from chemiluminescence to serological diagnosis of human toxoplasmosis].

作者信息

Fortier B, Dao A, Coignard-Chatain C, Biava M F

机构信息

Service de Parasitologie-Mycologie, Faculté de Médecine, Vandoeuvre-Les-Nancy, France.

出版信息

Pathol Biol (Paris). 1997 Nov;45(9):721-8.

PMID:9538470
Abstract

Diagnostic Products Corporation has chosen chemiluminescent for the new kit of quantitative measurement of IgG and qualitative detection of IgM antibodies to Toxoplasma gondii, 878 human sera of principal diagnosis situations were tested, and the results obtained with the IMMULITE Toxoplasmosis kit were compared with those of the Parasitology and Mycology Laboratory of the University of Lille. Chemiluminescent allows a sensitive and specific determination of immunity. In the same ways, this method is able to detect earlier specific IgM and IgG during seroconversion. The kit of quantitative measurement of IgG and qualitative detection of IgM is reproducible and sensitive; this confirms the interest for the pediatric diagnosis of congenital toxoplasmosis.

摘要

诊断产品公司已选择化学发光法用于新的试剂盒,以定量检测弓形虫IgG抗体及定性检测弓形虫IgM抗体。对878份处于主要诊断情形的人血清进行了检测,并将使用免疫发光法弓形虫病试剂盒获得的结果与里尔大学寄生虫学和真菌学实验室的结果进行了比较。化学发光法可实现对免疫情况的灵敏且特异的测定。同样,该方法能够在血清转化过程中更早地检测到特异性IgM和IgG。IgG定量检测及IgM定性检测试剂盒具有可重复性且灵敏;这证实了其在先天性弓形虫病儿科诊断中的价值。

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