Anwer M S, Gronwall R
Can J Physiol Pharmacol. 1976 Jun;54(3):277-86. doi: 10.1139/y76-041.
Bilirubin kinetics were studied in an isolated, perfused rat liver system using unconjugated (14C) bilirubin (UC(14C)B) and delta-amino (4-14 C) levulinic acid (A(14 C)LA) to derive a suitable compartmental model. Plasma disappearance of UC(14C)B, plasma appearance of conjugated (14c) bilirubin (C(14C)B) and biliary excretion of C(14C)B were followed for 90-120 min following injection of UC(14C)B. Hepatic content of labeled bilirubin 12 min after the injection of UC(14C)B was determined directly in five separate perfusion experiments. UCB was found to reflux back to plasma from liver in two experiments using A(14C)LA. Bilirubin binding to red blood cells (6-8% of the perfusate level) and the components of the perfusion apparatus (4-6% of perfusate level) was estimated by performing a control experiment without the liver. A six compartment model was necessary and adequate to explain the experimental data and current knowledge of bilirubin metabolism: (1) UCB bound to red blood cells and the perfusion apparatus, (2) plasma UCB, (3) liver UCB, (4) liver CB, (5) plasma CB, and (6) bile CB. The proposed model could serve as a reference point for studies of bilirubin kinetics in whole animals for normal and abnormal states.
使用未结合的（14C）胆红素（UC（14C）B）和δ-氨基（4-14C）乙酰丙酸（A（14C）LA）在离体灌注大鼠肝脏系统中研究胆红素动力学，以推导合适的房室模型。注射UC（14C）B后90 - 120分钟，追踪UC（14C）B的血浆消失、结合的（14C）胆红素（C（14C）B）的血浆出现以及C（14C）B的胆汁排泄。在五个独立的灌注实验中直接测定注射UC（14C）B后12分钟肝脏中标记胆红素的含量。在两个使用A（14C）LA的实验中发现未结合胆红素从肝脏回流到血浆中。通过进行无肝脏的对照实验来估计胆红素与红细胞的结合（灌注液水平的6 - 8%）以及灌注装置的成分（灌注液水平的4 - 6%）。一个六房室模型对于解释实验数据和当前胆红素代谢的知识是必要且足够的：（1）与红细胞和灌注装置结合的未结合胆红素，（2）血浆未结合胆红素，（3）肝脏未结合胆红素，（4）肝脏结合胆红素，（5）血浆结合胆红素，以及（6）胆汁结合胆红素。所提出的模型可作为研究正常和异常状态下全动物胆红素动力学的参考点。
