Takizawa T
Department of Anatomy, Jichi Medical School, Tochigi, Japan.
Kaibogaku Zasshi. 1998 Feb;73(1):1-7.
In this mini-review, we describe new enzyme cytochemical methodology combined with cryotechniques, which we have developed in order to reveal the ultrastructural localization of enzymes in biological membranes. This combined approach has been shown to be a valuable tool to study topology, dynamics, and function of enzymes in a more lifelike state.