Shahabuddin M, Gayle M, Zieler H, Laughinghouse A
Medical Entomology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Exp Parasitol. 1998 Feb;88(2):79-84. doi: 10.1006/expr.1998.4198.
We have developed a fluorescent labeling procedure for staining the mosquito stages of Plasmodium gallinaceum. PKH26, a lipophilic dye, is efficiently and permanently incorporated into the membranes of zygotes and ookinetes. Stained zygotes undergo normal development into ookinetes; the stain does not interfere with ookinete mobility or ability to adhere to the mosquito midgut lumen. Stained zygotes and ookinetes are comparable to untreated parasites in their ability to give rise to oocysts when fed to mosquitoes. This technique can be used to study the development of Plasmodium parasites in the complex cellular environment of the mosquito midgut after a blood meal. It may also be adapted to study other parasite-vector interactions.
我们已经开发出一种荧光标记程序，用于对鸡疟原虫在蚊子体内的各个阶段进行染色。PKH26是一种亲脂性染料，能有效且永久性地掺入合子和动合子的膜中。染色后的合子能正常发育为动合子；该染料不会干扰动合子的移动性或其黏附于蚊子中肠腔的能力。当将染色后的合子和动合子喂给蚊子时，它们产生卵囊的能力与未处理的寄生虫相当。这项技术可用于研究疟原虫在蚊虫吸食血液后中肠复杂细胞环境中的发育情况。它也可能适用于研究其他寄生虫与载体之间的相互作用。
