Torii M, Nakamura K, Sieber K P, Miller L H, Aikawa M
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
J Protozool. 1992 Jul-Aug;39(4):449-54. doi: 10.1111/j.1550-7408.1992.tb04830.x.
We observed Plasmodium gallinaceum ookinetes in both intracellular and intercellular positions in the midgut epithelium of the mosquito Aedes aegypti. After epithelial cell invasion intracellular ookinetes lacked a parasitophorous vacuolar membrane and were surrounded solely by their own pellicle. Thus, the ookinete in the midgut epithelium of the mosquito differs from erythrocytic and hepatic stages in that the parasite in the vertebrate host is surrounded by a vacuole. The midgut epithelial cytoplasm around the apical end of invading ookinetes was replaced by fine granular material deprived of normal organelles. Membranous structure was observed within the fine granular area. Most ookinetes were seen intracellularly on the luminal side and intercellularly on the haemocoel side of the midgut epithelial cells. These observations suggest that the ookinete first enters into the midgut epithelial cell, then exists to the space between the epithelial cells and moves to the basal lamina where the ookinete develops to the oocyst.
我们在埃及伊蚊中肠上皮细胞的细胞内和细胞间位置均观察到鸡疟原虫动合子。上皮细胞入侵后，细胞内动合子缺乏寄生泡膜，仅被其自身的表膜包围。因此，蚊子中肠上皮细胞中的动合子与红细胞期和肝期不同，因为脊椎动物宿主体内的寄生虫被液泡包围。入侵动合子顶端周围的中肠上皮细胞质被缺乏正常细胞器的细颗粒物质所取代。在细颗粒区域内观察到膜状结构。大多数动合子见于中肠上皮细胞腔面的细胞内和血腔面的细胞间。这些观察结果表明，动合子首先进入中肠上皮细胞，然后存在于上皮细胞之间的空间，并移动到基膜，在那里动合子发育成卵囊。
