Zanesi N, Mognato M, Pizzato M, Viezzer C, Ferri G, Celotti L
Dipartimento di Biologia, Università di Padova, Italy.
Mutat Res. 1998 Jan 30;412(2):177-86. doi: 10.1016/s1383-5718(97)00190-3.
We measured the frequency of mutant (MF) lymphocytes at the hprt locus in a population of 43 coke-oven workers exposed to PAH and in a group of 26 non-exposed workers. A non-significant increase in MF in the exposed group (19.0 +/- 16.3) compared to the non-exposed group (15.8 +/- 14.6) was observed. Moreover, when we considered smoking habits for the overall population, the MF values were higher, although not significantly, in smokers than in non-smokers. For some T-cell mutant clone structural alterations, splicing and coding errors were detected by PCR-based methods. We analysed 161 HPRT- clones, derived from exposed and non-exposed workers by multiplex-PCR and 56 HPRT- clones by reverse transcriptase-PCR. Overall, the percentages of the different types of gene alterations were similar in exposed and non-exposed subjects. Only the frequency of splice mutations in mutant clones derived from coke-oven workers was higher (22%) than in non-exposed donors (11%).
我们测量了43名接触多环芳烃的焦炉工人和26名未接触工人群体中次黄嘌呤-鸟嘌呤磷酸核糖转移酶（hprt）基因座处突变（MF）淋巴细胞的频率。与未接触组（15.8±14.6）相比，接触组（19.0±16.3）的MF有不显著增加。此外，当我们考虑总体人群的吸烟习惯时，吸烟者的MF值虽不显著但高于非吸烟者。对于一些T细胞突变克隆结构改变，通过基于聚合酶链反应（PCR）的方法检测到了剪接和编码错误。我们通过多重PCR分析了来自接触和未接触工人的161个HPRT-克隆，通过逆转录PCR分析了56个HPRT-克隆。总体而言，接触和未接触受试者中不同类型基因改变的百分比相似。仅来自焦炉工人的突变克隆中剪接突变的频率（22%）高于未接触供体（11%）。
