Kariya N
Department of Anesthesiology and Intensive Care Medicine, Osaka City University Medical School, Japan.
Osaka City Med J. 1997 Dec;43(2):243-51.
We studied the effect of amrinone on portal perfusion pressure, perfusion flow, and tissue blood flow using an isolated perfused rat liver model. In the constant perfusion flow model, amrinone effectively decreased perfusion pressure in the precontracted state by adenosine triphosphate (ATP) or norepinephrine. Amrinone dose-dependently decreased portal perfusion pressure increased by calcium chloride. Similarly, amrinone dose-dependently increased portal perfusion flow decreased by ATP in the constant perfusion pressure model. Amrinone effectively increased tissue blood flow decreased by ATP or norepinephrine measured by laser-Doppler flowmetry. A specific inhibitor of the biosynthesis of nitric oxide, N omega-nitro-L-arginine, did not affect the hemodynamic effect of amrinone, suggesting that nitric oxide is not involved in the portal vasodilating effect of amrinone. We conclude that amrinone increases portal blood flow by decreasing perfusion pressure and contributes to increasing tissue blood flow of the liver without the involvement of nitric oxide.
我们使用离体灌注大鼠肝脏模型研究了氨力农对门静脉灌注压、灌注流量和组织血流的影响。在恒定灌注流量模型中，氨力农可有效降低由三磷酸腺苷（ATP）或去甲肾上腺素所致预收缩状态下的灌注压。氨力农剂量依赖性地降低氯化钙所致升高的门静脉灌注压。同样，在恒定灌注压模型中，氨力农剂量依赖性地增加ATP所致降低的门静脉灌注流量。氨力农可有效增加经激光多普勒血流仪测定的由ATP或去甲肾上腺素所致降低的组织血流。一氧化氮生物合成的特异性抑制剂Nω-硝基-L-精氨酸不影响氨力农的血流动力学效应，提示一氧化氮不参与氨力农的门静脉舒张效应。我们得出结论，氨力农通过降低灌注压增加门静脉血流，并在不涉及一氧化氮的情况下有助于增加肝脏组织血流。
