Doel M T, Carey N H
Cell. 1976 May;8(1):51-8. doi: 10.1016/0092-8674(76)90184-7.
We present evidence that the poly(A) sequence at the 3' end of ovalbumin mRNA has an effect on its translational efficiency in a reticulocyte lysate cell-free system. Polynucleotide phosphorylase has been used to remove selectively the poly(A) while leaving the rest of the molecule intact. It is shown that the stability of the mRNA in a cell free system is not appreciably affected by this procedure. Measurements of the size of ovalbumin-synthesizing polysomes, rate of peptide elongation, and number of rounds of translation per messenger show a generally reduced efficiency for deadenylated mRNA compared to native mRNA. No comparable difference was observed in experiments with a wheat germ cell-free system, which gives few rounds of translation per mRNA. This indicates that the effect results from a lowering of the efficiency of reinitiation on deadenylated mRNA.
我们提供的证据表明，卵清蛋白mRNA 3'端的多聚腺苷酸（poly(A)）序列对其在网织红细胞裂解物无细胞系统中的翻译效率有影响。已使用多核苷酸磷酸化酶选择性地去除poly(A)，同时使分子的其余部分保持完整。结果表明，该过程对无细胞系统中mRNA的稳定性没有明显影响。对合成卵清蛋白的多核糖体大小、肽链延伸速率以及每个信使RNA的翻译轮数的测量表明，与天然mRNA相比，去腺苷酸化的mRNA的效率普遍降低。在小麦胚无细胞系统的实验中未观察到类似差异，该系统每个mRNA的翻译轮数很少。这表明这种影响是由于去腺苷酸化的mRNA重新起始效率降低所致。
