Wasmuth J J, Caskey C T
Cell. 1976 May;8(1):71-7. doi: 10.1016/0092-8674(76)90187-2.
We have isolated variants of Chinese hamster lung (CHL) fibroblasts with decreased sensitivity to the toxic proline analog, L-azetidine-2-carboxylic acid (AZCA). Resistance to AZCA is a stable characteristic of these cell lines since it is maintained after 40 generations in nonselective medium. Two AZCA-resistant cell lines overproduce and excrete more proline than wild-type cells. Furthermore, AZCA rapidly inhibits the synthesis of proline from glutamic acid in wild-type but not the resistant cell lines. These studies suggest that resistance to AZCA in these cell lines is due to an alteration in some component of the proline biosynthetic pathway, possibly a regulatory site that is inhibited by AZCA.
我们已经分离出对毒性脯氨酸类似物L-氮杂环丁烷-2-羧酸（AZCA）敏感性降低的中国仓鼠肺（CHL）成纤维细胞变体。对AZCA的抗性是这些细胞系的稳定特征，因为在非选择性培养基中培养40代后仍能保持。与野生型细胞相比，两个抗AZCA的细胞系过量产生并分泌更多的脯氨酸。此外，AZCA能迅速抑制野生型细胞中由谷氨酸合成脯氨酸的过程，但对抗性细胞系没有影响。这些研究表明，这些细胞系对AZCA的抗性是由于脯氨酸生物合成途径中某些成分的改变，可能是一个被AZCA抑制的调节位点。
