Spohr G, Dettori G, Manzari V
Cell. 1976 Aug;8(4):505-12. doi: 10.1016/0092-8674(76)90218-x.
Nuclear RNA from immature duck erythrocytes was fractionated into polyadenylated and nonpolyadenylated fractions, and globin mRNA sequences were determined by hybridization to DNA complementary to globin mRNA. 80--90% of labeled nuclear RNA is found to be nonpolyadenylated, and 70--80% of the globin mRNA sequences present in the nucleus are found in nonpolyadenylated molecules. These data suggest that polyadenylation does not specifically select for globin mRNA sequences. The nonpolyadenylated globin mRNA sequences present in the nucleus are found mostly in molecules of small size, close to the size of polyribosomal globin mRNA, suggesting that polyadenylation is a later event in globin mRNA formation.
将未成熟鸭红细胞的核RNA分离为聚腺苷酸化和非聚腺苷酸化组分，并通过与球蛋白mRNA互补的DNA杂交来确定球蛋白mRNA序列。发现80%-90%的标记核RNA是非聚腺苷酸化的，并且存在于细胞核中的70%-80%的球蛋白mRNA序列存在于非聚腺苷酸化分子中。这些数据表明聚腺苷酸化并非特异性地选择球蛋白mRNA序列。存在于细胞核中的非聚腺苷酸化球蛋白mRNA序列大多存在于小尺寸分子中，接近多核糖体球蛋白mRNA的大小，这表明聚腺苷酸化是球蛋白mRNA形成过程中的后期事件。
