鸭珠蛋白信使核糖核酸前体原位交联的电子显微镜研究
Electron microscope study of duck globin mRNA precursor crosslinked in situ.
作者信息
Reymond C, Aglianó A, Spohr G
出版信息
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4683-7. doi: 10.1073/pnas.77.8.4683.
Abstract
Double-stranded RNA segments present in duck globin pre-mRNA were crosslinked in situ with aminomethyltrioxalen and UV light. The secondary structure of the crosslinked pre-mRNA was then studied by electron-microscopic analysis of pre-mRNA . cDNA hybrids. The data suggest that duck globin pre-mRNAs contain intervening sequences that are excised stepwise. Excision and subsequent ligation appears to occur on precursor molecules that are stabilized by base-paired regions.
摘要
鸭珠蛋白前体信使核糖核酸（pre-mRNA）中存在的双链核糖核酸片段，通过氨甲基三氧杂蒽和紫外线进行原位交联。然后，通过对前体信使核糖核酸.cDNA杂交体进行电子显微镜分析，研究交联前体信使核糖核酸的二级结构。数据表明，鸭珠蛋白前体信使核糖核酸含有逐步切除的间隔序列。切除和随后的连接似乎发生在由碱基配对区域稳定的前体分子上。
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