甲状旁腺激素相关蛋白在小鼠磨牙发育中的作用
Developmental role of PTHrP in murine molars.
作者信息
Liu J G, Tabata M J, Yamashita K, Matsumura T, Iwamoto M, Kurisu K
机构信息
Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, Yamadaoka, Suita, Japan.
出版信息
Eur J Oral Sci. 1998 Jan;106 Suppl 1:143-6. doi: 10.1111/j.1600-0722.1998.tb02167.x.
Although PTHrP is produced in multiple fetal tissues, its precise physiological functions have yet to be clearly elucidated. The present study was undertaken to elucidate the biological role of PTHrP in the development of tooth. In rat tooth germs, the PTHrP and its receptor genes were expressed in the enamel organ and dental mesenchyme, respectively. When mouse tooth explants were cultured with antisense oligodeoxyribonucleotides (ODN) against mouse PTHrP mRNA in serum-free medium, an invasion of bone tissue was observed in the tooth germs. On the other hand, the explants cultured without ODN or with sense ODN showed normal histological structures similar to those observed in vivo. These results suggest that PTHrP is essential for tooth development and for the protection of tooth germs from the invasion of bone tissue.
尽管甲状旁腺激素相关蛋白（PTHrP）在多种胎儿组织中产生，但其确切的生理功能尚未得到明确阐明。本研究旨在阐明PTHrP在牙齿发育中的生物学作用。在大鼠牙胚中，PTHrP及其受体基因分别在成釉器和牙间充质中表达。当在无血清培养基中用针对小鼠PTHrP mRNA的反义寡脱氧核糖核苷酸（ODN）培养小鼠牙外植体时，在牙胚中观察到骨组织的侵入。另一方面，未用ODN或用正义ODN培养的外植体显示出与体内观察到的相似的正常组织结构。这些结果表明，PTHrP对于牙齿发育以及保护牙胚免受骨组织侵入至关重要。
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