Embery G, Rees S, Hall R, Rose K, Waddington R, Shellis P
Department of Basic Dental Science, Dental School, University of Wales College of Medicine, Cardiff, UK.
Eur J Oral Sci. 1998 Jan;106 Suppl 1:267-73. doi: 10.1111/j.1600-0722.1998.tb02186.x.
This study describes the interaction of a small chondroitin sulphate proteoglycan and the glycosaminoglycans chondroitin 4-sulphate, dermatan sulphate and heparan sulphate with hydroxyapatite. All macromolecules possessed a high affinity, with the iduronic acid-rich dermatan sulphate and heparan sulphate displaying higher adsorption maxima than the glucuronic acid-rich chondroitin 4-sulphate. At similar concentrations, dermatan sulphate produced a 30% inhibition of hydroxyapatite-induced crystal growth, whilst chondroitin 4-sulphate yielded 50% inhibition. Estimation of the calcium binding capacity of these glycosaminoglycans using equilibrium dialysis indicated that chondroitin 4-sulphate bound five times more calcium than dermatan sulphate at a calcium concentration similar to that of serum. The data indicate a possible important role for chondroitin 4-sulphate in dentinogenesis where it is the dominant glycosaminoglycan, since it could act as a capture point for calcium ions during mineralisation, with the leucine-rich domain of its parent proteoglycan acting as anchor points to type I collagen.
本研究描述了一种小硫酸软骨素蛋白聚糖以及硫酸软骨素4-硫酸酯、硫酸皮肤素和硫酸乙酰肝素等糖胺聚糖与羟基磷灰石的相互作用。所有大分子均具有高亲和力，富含艾杜糖醛酸的硫酸皮肤素和硫酸乙酰肝素比富含葡萄糖醛酸的硫酸软骨素4-硫酸酯表现出更高的吸附最大值。在相似浓度下，硫酸皮肤素对羟基磷灰石诱导的晶体生长产生30%的抑制作用，而硫酸软骨素4-硫酸酯产生50%的抑制作用。使用平衡透析法对这些糖胺聚糖的钙结合能力进行评估表明，在与血清钙浓度相似的情况下，硫酸软骨素4-硫酸酯结合的钙是硫酸皮肤素的五倍。数据表明硫酸软骨素4-硫酸酯在牙本质形成过程中可能发挥重要作用，因为它是牙本质中主要的糖胺聚糖，在矿化过程中它可作为钙离子的捕获点，其母体蛋白聚糖富含亮氨酸的结构域作为与I型胶原的锚定点。
