Mishima H, Kozawa Y
Department of Anatomy, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Eur J Oral Sci. 1998 Jan;106 Suppl 1:392-6. doi: 10.1111/j.1600-0722.1998.tb02204.x.
The present study was designed to characterize the morphology and composition of calcospherites in the coronal and root predentin of human permanent teeth by scanning electron microscopy (SEM) and scanning electron microscopy energy dispersive spectroscopy (SEM-EDS). Human incisors, premolars, and molars were used. The calcospherites in the coronal predentin were globular and 10-20 microm in diameter. The calcospherites in the root predentin were smaller and their shape was different. Polygonal calcospherites and stellate calcospherites were observed in the intermediate region of the root predentin. Calcospherites were rarely present in the apical region of the root predentin. Calcified matrix fibers were observed in the apical region of the root predentin. The Ca/P molar ratio in crown calcospherites (1.63 +/- 0.27) differed significantly from that in root calcospherites (1.46 +/- 0.28). Sulfur was detected from the cervical region to the root region, but not in the horn region. Odontoblast activity and the local environment of the predentin are thought to determine the shape, size, and composition of calcospherites.
本研究旨在通过扫描电子显微镜（SEM）和扫描电子显微镜能谱仪（SEM-EDS）对人类恒牙冠部和根部前期牙本质中钙球的形态和成分进行表征。使用了人类的切牙、前磨牙和磨牙。冠部前期牙本质中的钙球呈球形，直径为10-20微米。根部前期牙本质中的钙球较小，且形状不同。在根部前期牙本质的中间区域观察到多边形钙球和星状钙球。在根部前期牙本质的根尖区域很少有钙球存在。在根部前期牙本质的根尖区域观察到钙化的基质纤维。冠部钙球中的钙磷摩尔比（1.63±0.27）与根部钙球中的钙磷摩尔比（1.46±0.28）有显著差异。从颈部区域到根部区域检测到了硫，但在角部区域未检测到。成牙本质细胞活性和前期牙本质的局部环境被认为决定了钙球的形状、大小和成分。
