蜘蛛小壶腹丝蛋白包含新的重复序列和高度保守的非丝样“间隔区”。
Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved non-silk-like "spacer regions".
作者信息
Colgin M A, Lewis R V
机构信息
Department of Molecular Biology, University of Wyoming, Laramie 82071-3944, USA.
出版信息
Protein Sci. 1998 Mar;7(3):667-72. doi: 10.1002/pro.5560070315.
Abstract
Spider minor ampullate silk is a strong non-elastic deformably stretchable silk used in web formation. This silk from Nephila clavipes is composed of two proteins, MiSp 1 and 2, whose transcripts are 9.5 and 7.5 kb, respectively, as determined by Northern blots. Both MiSp proteins are organized into a predominantly repetitive region and a small nonrepetitive carboxy terminal region. These highly repetitive regions are composed mainly of glycine and alanine, but also contain tyrosine, glutamine, and arginine. The sequences are mainly GGX and GA repeats. The repetitive regions are interrupted by nonrepetitive serine-rich spacer regions. Although the sequences of the spacer regions differ from the repetitive regions, sequences of the spacers from different regions of the proteins are nearly identical. The sequence differences between major and minor ampullate silks may explain the differing mechanical properties of the fibers.
摘要
小壶腹蛛丝是一种用于织网的高强度、无弹性、可变形拉伸的蛛丝。这种来自金蛛属蜘蛛的蛛丝由两种蛋白质MiSp 1和MiSp 2组成，通过Northern印迹法测定，它们的转录本分别为9.5 kb和7.5 kb。两种MiSp蛋白质都由一个主要的重复区域和一个小非重复的羧基末端区域组成。这些高度重复的区域主要由甘氨酸和丙氨酸组成，但也含有酪氨酸、谷氨酰胺和精氨酸。序列主要是GGX和GA重复序列。重复区域被富含丝氨酸的非重复间隔区域打断。尽管间隔区域的序列与重复区域不同，但来自蛋白质不同区域的间隔序列几乎相同。大壶腹蛛丝和小壶腹蛛丝之间的序列差异可能解释了纤维不同的机械性能。
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