pH 依赖的可溶性单体流感血凝素的聚集与分泌
pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin.
作者信息
Vanlandschoot P, Beirnaert E, Grooten J, Jou W M, Fiers W
机构信息
Laboratory of Molecular Biology, Flanders Interuniversity Institute of Biotechnology, University of Ghent, Belgium.
出版信息
Arch Virol. 1998;143(2):227-39. doi: 10.1007/s007050050282.
We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a monomeric and possibly a trimeric structure is detected in cell supernatant, irrespective of the pH of the medium. Evidence is presented that the aggregated fraction is generated out of monomeric HAOs molecules due to a low intracellular pH encountered during secretion.
我们之前报道了可溶性A/维多利亚/3/75（H3N2）血凝素在昆虫细胞中的表达以及聚集部分的分子和免疫结构，只有在低pH条件下进行表达时才会在细胞上清液中观察到这种聚集部分。在此我们报道，除了这种聚集结构外，无论培养基的pH值如何，在细胞上清液中还检测到了单体结构以及可能的三聚体结构。有证据表明，由于分泌过程中细胞内pH值较低，聚集部分是由单体HAOs分子形成的。
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