Ikeda K, Nakazawa H, Alain R, Belloncik S, Mori H
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Japan.
Arch Virol. 1998;143(2):241-8. doi: 10.1007/s007050050283.
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) polyhedrin gene from natural mutant strain B, B2 and P was cloned and the amino acid sequence was compared with that of wild-type virus (strain H). Chimeric polyhedrin genes (8 types) containing only one site of mutation within the amino acid sequence were also constructed. Fifteen types of polyhedrin genes were introduced into a baculovirus expression vector and hexahedral, acicular, pyramidal, or amorphous polyhedra were formed in infected cells. These results demonstrated that the shape of polyhedra as well as the crystallization pattern of the polyhedrin could be changed by mutations at respectively N-terminal and C-terminal regions of BmCPV polyhedrin gene.
克隆了来自天然突变株B、B2和P的家蚕细胞质多角体病毒（BmCPV）多角体蛋白基因，并将其氨基酸序列与野生型病毒（H株）的进行了比较。还构建了在氨基酸序列中仅含有一个突变位点的嵌合多角体蛋白基因（8种）。将15种多角体蛋白基因导入杆状病毒表达载体，在感染细胞中形成了六面体、针状、金字塔状或无定形多面体。这些结果表明，BmCPV多角体蛋白基因的N端和C端区域的突变分别可以改变多面体的形状以及多角体蛋白的结晶模式。
