Gong Y, Shannon A, Westaway E G, Gowans E J
Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston, Brisbane, Australia.
Arch Virol. 1998;143(2):399-404. doi: 10.1007/s007050050296.
The replication of bovine viral diarrhoea virus (BVDV) RNA is considered to involve replicative intermediates (RI) and replicative forms (RF) of virus RNA. These RNA species were radiolabelled metabolically by 3H-uridine in BVDV-infected cells and separated by sucrose gradient centrifugation. RNA in the fractions was then digested with RNase A in high salt (2 x SSC) and the RNase-resistance was determined. Using the Baltimore formula, this led to the calculation that the RI of BVDV contained 6-7 nascent strands on the template. This suggests that the structure of the BVDV RI is similar to that of poliovirus and dengue 2 virus.
牛病毒性腹泻病毒（BVDV）RNA的复制被认为涉及病毒RNA的复制中间体（RI）和复制形式（RF）。这些RNA种类在BVDV感染的细胞中通过3H-尿苷进行代谢性放射性标记，并通过蔗糖梯度离心进行分离。然后将各组分中的RNA在高盐（2×SSC）条件下用核糖核酸酶A消化，并测定核糖核酸酶抗性。使用巴尔的摩公式，由此计算出BVDV的RI在模板上含有6 - 7条新生链。这表明BVDV RI的结构与脊髓灰质炎病毒和登革热2型病毒的结构相似。
