Iwanami T, Kondo Y, Makita Y, Azeyanagi C, Ieki H
Department of Citriculture, National Institute of Fruit Tree Science, Shizuoka, Japan.
Arch Virol. 1998;143(2):405-12. doi: 10.1007/s007050050297.
The sequence of the 3'-terminal 4320 and 2409 nucleotides were determined for RNA2 of satsuma dwarf virus (SDV) and navel infectious mottling virus (NIMV). Both sequences contained a part of a long open reading frame which encodes larger and smaller coat proteins (CPs) at the 3'-terminus followed by a 3'non-coding region upstream of a poly (A) tail. Amino acid sequence identity for larger and smaller CPs ranged 81-84% and 68-78%, respectively, among SDV, NIMV and the previously sequenced citrus mosaic virus (CiMV). No significant sequence similarity was found between the CPs of SDV or NIMV and those of the como-, nepo- or other viruses. The nucleotide sequence identity of the 3' non-coding region of RNA2 were 68%-78% among SDV, CiMV and NIMV. These results suggest that SDV, CiMV and NIMV are distinct, though related, viruses. They may be assigned as members of the new genus, which is close to the genera of Comovirus and Nepovirus.
测定了温州蜜柑萎缩病毒（SDV）和脐橙传染性斑驳病毒（NIMV）RNA2的3'末端4320和2409个核苷酸的序列。两个序列均包含一个长开放阅读框的一部分，该阅读框在3'末端编码大小不同的外壳蛋白（CPs），随后是聚（A）尾上游的3'非编码区。在SDV、NIMV和先前测序的柑橘花叶病毒（CiMV）中，大小CPs的氨基酸序列同一性分别为81-84%和68-78%。在SDV或NIMV的CPs与豇豆花叶病毒属、线虫传多面体病毒属或其他病毒的CPs之间未发现明显的序列相似性。RNA2的3'非编码区的核苷酸序列同一性在SDV、CiMV和NIMV之间为68%-78%。这些结果表明，SDV、CiMV和NIMV是不同但相关的病毒。它们可能被归为一个新属的成员，该新属与豇豆花叶病毒属和线虫传多面体病毒属相近。
