Kintz P, Cirimele V, Ludes B
Institut de Médecine Légale, Strasbourg, France.
Int J Legal Med. 1998;111(2):82-4. doi: 10.1007/s004140050119.
With the growing interest in drug testing within different sectors of society, there has become a need for drug assays that can be performed immediately at the site of specimen collection. Recently, Securetec (Ottobrunn, Germany) has introduced the Drugwipe, a non instrument-based, on-site immunodiagnostic assay for the detection of drugs on surfaces. Different tests are available for opiates, cocaine and cannabis. To document the applications of the Drugwipe "opiate" on human biological fluids, 60 mg codeine phosphate were orally administered to 6 subjects. First, sweat testing with the Drugwipe was studied. The wiping section of the kit was used to swab the forehead of the subjects for 10 s, at 1, 4, 9 and 24 h after codeine administration. At the same time, for each period, a sweat patch (Pharmchek, USA) was applied to the outer portion of the upper arm. Codeine was then quantified in the patch by GC/MS and the measured concentrations used as reference. In all subjects except one the Drugwipe tested positive for opiates, however with few false negative results. In the second part of the study, results of the Drugwipe were compared with those obtained by GC/MS for saliva. The tongue of the subjects was carefully wiped over a period 24 h, and at the same time a specimen of saliva collected. Although codeine could be detected using the Drugwipe, numerous false negative results were observed. Codeine tested positive by GC/MS but remained negative using the Drugwipe in several cases. This can be explained by a codeine concentration which was too low to show positive with the Drugwipe, interfering substances may be present in saliva or the sampling procedure is inadequate.
随着社会不同领域对药物检测的兴趣日益浓厚，对能够在样本采集现场立即进行的药物检测方法的需求也随之增加。最近，德国奥托布伦的Securetec公司推出了Drugwipe，这是一种基于非仪器的现场免疫诊断检测方法，用于检测表面的药物。有针对阿片类药物、可卡因和大麻的不同检测方法。为了记录Drugwipe“阿片类药物”在人体生物体液中的应用情况，向6名受试者口服了60毫克磷酸可待因。首先，研究了使用Drugwipe进行汗液检测。在服用可待因后的1小时、4小时、9小时和24小时，使用试剂盒的擦拭部分在受试者的前额擦拭10秒。同时，在每个时间段，将一个汗液贴片（美国Pharmchek）贴在上臂外侧。然后通过气相色谱/质谱法对贴片中的可待因进行定量，并将测量浓度用作参考。除一名受试者外，所有受试者的Drugwipe检测阿片类药物均呈阳性，但假阴性结果较少。在研究的第二部分，将Drugwipe的结果与通过气相色谱/质谱法检测唾液获得的结果进行了比较。在24小时内仔细擦拭受试者的舌头，同时采集一份唾液样本。尽管使用Drugwipe可以检测到可待因，但观察到了许多假阴性结果。在一些情况下，可待因通过气相色谱/质谱法检测呈阳性，但使用Drugwipe检测仍为阴性。这可能是由于可待因浓度过低，无法使Drugwipe显示阳性，唾液中可能存在干扰物质，或者采样程序不充分。
