Portillo F, Carrasco M, Vallo J J
Departamento de Fisiología, Facultad de Medicina, Cádiz, Spain.
Rev Esp Fisiol. 1997 Dec;53(4):361-6.
Anorexia inducing lithium chloride is believed to involve descending projections from hypothalamus to preganglionic autonomic output neurons. A multiple-labelling technique has presently been used to analyze the anatomical projections of lithium chloride sensitive neurons in the hypothalamus. Immunolabelling of c-fos was performed to stain neurons activated after LiCl administration, while neurons projecting toward vagal parasympathetic preganglionic levels were identified by injection of diamidino yellow in the dorsal motor nucleus of the vagus. Perikarya of descending neurons were mainly observed in the ventral and lateral areas of the paraventricular hypothalamic nucleus. In contrast, lithium chloride activated neurons were observed mainly in the magnocellular division of the paraventricular nucleus and supraoptic nucleus. Double-labelled neurons were not observed. These data provide evidence that lithium chloride sensitive neurons in the paraventricular nucleus are clearly different from those descending toward preganglionic vagal outflow neurons.
据信，诱发厌食的氯化锂涉及从下丘脑到节前自主输出神经元的下行投射。目前已使用多重标记技术来分析下丘脑中对氯化锂敏感的神经元的解剖投射。通过对c-fos进行免疫标记来对氯化锂给药后激活的神经元进行染色，而通过向迷走神经背运动核注射双脒基黄来识别向迷走神经副交感节前水平投射的神经元。下行神经元的胞体主要见于下丘脑室旁核的腹侧和外侧区域。相比之下，氯化锂激活的神经元主要见于室旁核和视上核的大细胞部。未观察到双标记神经元。这些数据证明，室旁核中对氯化锂敏感的神经元与那些向下投射至迷走神经节前传出神经元的神经元明显不同。
