针对解脲脲原体9型血清型抗原的单克隆抗体的研制。
Development of a monoclonal antibody to a Ureaplasma urealyticum serotype 9 antigen.
作者信息
Naessens A, Cheng X, Lauwers S, Robertson J A
机构信息
Department of Microbiology, Akademisch Ziekenhuis, Vrije Universiteit Brussel, Brussels, Belgium.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1125-7. doi: 10.1128/JCM.36.4.1125-1127.1998.
Abstract
We produced a monoclonal antibody (MAb) to Ureaplasma urealyticum Vancouver, the serotype 9 standard strain. By immunoblotting, this MAb showed a single, 85-kDa band with the homologous serotype and a minor, 100-kDa band with serotype 2 but did not react with any other serotype standard strain. Clinical isolates of U. urealyticum were tested with this MAb and with two sets of polyclonal antisera against the 14 serotype standard strains. The use of MAb 9-2H9 correctly identified certain serotype 9 strains but did not react with wild-type strains lacking the serotype 9 determinant.
摘要
我们制备了一种针对解脲脲原体温哥华株（血清型9标准菌株）的单克隆抗体（MAb）。通过免疫印迹法，该单克隆抗体与同源血清型显示出一条单一的85 kDa条带，与血清型2显示出一条较小的100 kDa条带，但不与任何其他血清型标准菌株发生反应。用该单克隆抗体以及两组针对14种血清型标准菌株的多克隆抗血清对解脲脲原体的临床分离株进行了检测。单克隆抗体9 - 2H9的使用正确鉴定了某些血清型9菌株，但不与缺乏血清型9决定簇的野生型菌株发生反应。
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3
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