Yasue M, Yokota T, Yuasa M, Kajiwara Y, Suko M, Okudaira H
Bioscience Research and Development Laboratory, Asahi Breweries Ltd., Ibaraki, Japan.
Eur Respir J. 1998 Jan;11(1):144-50. doi: 10.1183/09031936.98.11010144.
Recombinant Der f2 (rDer f2) has recently been developed as a promising allergen for the diagnosis and immunotherapy of house-dust mite allergy, and studies in immunology. The aim of the present study was to evaluate whether oral administration of rDer f2 could suppress an immediate allergic reaction in mice sensitized with mite allergen. We developed a murine allergic model that showed bronchoconstriction after inhalation of rDer f2, and studied the effect of oral administration of rDer f2 on the reaction. Seven week old male A/J mice were intranasally immunized with rDer f2 12 times. Sensitized mice showed anti-rDer f2 immunoglobulin (Ig)E production and immediate airway constriction after inhalation of 10 mg.mL(-1) of rDer f2, as determined by the Konzett-Rössler method. Immunized animals were divided into three groups, and fed phosphate-buffered saline (PBS), 0.1 mg.day(-1), or 1 mg.day(-1) of rDer f2 for 4 weeks, respectively. Seven days after the last feeding, the mice were examined for their immediate response. Animals fed with 1 mg.day(-1) rDer f2 showed significantly reduced bronchoconstriction after inhalation of both 2 mg.mL(-1) and 10 mg.mL(-1) of rDer f2 compared with PBS-fed mice. Similar results were obtained when we examined mice 10 weeks after the last feeding. Reactions in the 0.1 mg.day(-1) rDer f2-fed group also tended to decrease in comparison with PBS-fed animals. Plasma anti-rDer f2 IgE, IgG1, IgG2a, and IgG2b levels were not changed by feeding with rDer f2. We conclude that recombinant Der f2 exhibits both sensitizing and hyposensitizing activities in mice. rDer f2 may be useful in immunotherapy and diagnosis of house-dust mite allergy.
重组Der f2（rDer f2）最近已被开发成为一种用于屋尘螨过敏诊断和免疫治疗以及免疫学研究的有前景的变应原。本研究的目的是评估口服rDer f2是否能抑制用螨变应原致敏的小鼠的速发型过敏反应。我们建立了一种小鼠过敏模型，该模型在吸入rDer f2后出现支气管收缩，并研究了口服rDer f2对该反应的影响。7周龄雄性A/J小鼠用rDer f2进行鼻内免疫12次。通过Konzett-Rössler方法测定，致敏小鼠在吸入10 mg·mL⁻¹的rDer f2后显示出抗rDer f2免疫球蛋白（Ig）E产生和即刻气道收缩。将免疫动物分为三组，分别喂食磷酸盐缓冲盐水（PBS）、0.1 mg·天⁻¹或1 mg·天⁻¹的rDer f2，持续4周。最后一次喂食7天后，检查小鼠的即刻反应。与喂食PBS的小鼠相比，喂食1 mg·天⁻¹ rDer f2的动物在吸入2 mg·mL⁻¹和10 mg·mL⁻¹的rDer f2后支气管收缩明显减轻。在最后一次喂食10周后检查小鼠时也获得了类似结果。与喂食PBS的动物相比，喂食0.1 mg·天⁻¹ rDer f2组的反应也有降低趋势。喂食rDer f2后血浆抗rDer f2 IgE、IgG1、IgG2a和IgG2b水平未发生变化。我们得出结论，重组Der f2在小鼠中表现出致敏和减敏活性。rDer f2可能在屋尘螨过敏的免疫治疗和诊断中有用。
