Santoreneos S, Stoodley M A, Jones N R, Brown C J
Department of Neurosurgery, Royal Adelaide Hospital, University of Adelaide, North Terrace, SA, Australia.
J Neurosci Methods. 1998 Feb 20;79(2):195-9. doi: 10.1016/s0165-0270(97)00180-5.
A method for perfusion fixation of the sheep central nervous system is described. The technique utilises the vascular anatomy of the spinal cord and brain to provide fixation that is comparable to transcardiac perfusion of the small animal. It can be applied with relative ease, cost effectiveness and without risking premature death of the animal due to complications of a major procedure such as thoracotomy.
本文描述了一种用于绵羊中枢神经系统灌注固定的方法。该技术利用脊髓和脑的血管解剖结构,以提供与小动物经心灌注相当的固定效果。它可以相对容易地应用,具有成本效益,并且不会因诸如开胸术等大型手术的并发症而导致动物过早死亡。