Belgrader P, Smith J K, Weedn V W, Northrup M A
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.
J Forensic Sci. 1998 Mar;43(2):315-9.
A microfabricated, battery-powered thermal cycler was implemented in PCR-based DNA typing for human identification. HLA DQ alpha and an STR triplex were PCR amplified using a device known as the Miniature Analytical Thermal Cycling Instrument (MATCI). The extremely efficient heating properties of the MATCI enabled thermal cycling to be completed in as little as 21 min. In addition, the feasibility of using the real-time fluorescent detection system of the MATCI was demonstrated. The successful application of this portable, prototype device to forensic identity testing is a significant milestone towards the eventual development of a completely integrated DNA testing instrument that would also incorporate sample preparation and allele detection.
一种微型的、电池供电的热循环仪被应用于基于聚合酶链反应（PCR）的人类身份鉴定DNA分型中。使用一种名为微型分析热循环仪（MATCI）的设备对人类白细胞抗原DQα（HLA DQ alpha）和一个短串联重复序列三联体进行了PCR扩增。MATCI极其高效的加热特性使得热循环能够在短短21分钟内完成。此外，还证明了使用MATCI实时荧光检测系统的可行性。这种便携式原型设备在法医身份鉴定测试中的成功应用，是朝着最终开发出一种完全集成的DNA检测仪器迈出的重要里程碑，该仪器还将包括样品制备和等位基因检测功能。
