Hanaoka Y, Minaguch K
Department of Forensic Odontology, Tokyo Dental College, Chiba, Japan.
J Forensic Sci. 1998 Mar;43(2):406-9.
VNTR polymorphism at the D4S43 locus was investigated, and allele frequencies in the Japanese population sample were determined by the polymerase chain reaction. Eleven different alleles and 16 genotypes were observed in 131 unrelated Japanese. The most common allele was one repeat unit (60.3%), the heterozygosity index of the present study was 58.7%, and the polymorphic information content was calculated at 0.55. In addition, we found four new size variations in the one-repeat-unit allele, which was the most common allele among the Japanese population. These variations were believed to be different from variations by repeats of the basic 14-bp unit. The one-repeat-unit allele at the D4S43 locus, which comes in four sizes, was detected in all of the samples, including degraded DNA samples obtained from hard tooth tissue. Therefore, this new variation is useful in personal identification by DNA analysis using forensic scientific degraded DNA samples.
研究了D4S43位点的VNTR多态性，并通过聚合酶链反应确定了日本人群样本中的等位基因频率。在131名无关的日本人中观察到11种不同的等位基因和16种基因型。最常见的等位基因是一个重复单元（60.3%），本研究的杂合度指数为58.7%，多态信息含量计算为0.55。此外，我们在最常见的一个重复单元等位基因中发现了四个新的大小变异，这些变异被认为与基本14bp单元重复产生的变异不同。在所有样本中都检测到了D4S43位点的一个重复单元等位基因，其有四种大小，包括从硬齿组织获得的降解DNA样本。因此，这种新变异在使用法医科学降解DNA样本进行DNA分析的个人识别中很有用。
