Chevolot L, Colliec-Jouault S, Foucault A, Ratiskol J, Sinquin C
URM2-CNRS URA 502, Laboratoire de recherche sur les macromolécules, Université Paris Nord, Villetaneuse, France.
J Chromatogr B Biomed Sci Appl. 1998 Feb 27;706(1):43-54. doi: 10.1016/s0378-4347(97)00448-9.
A new method combining ion-exchange displacement chromatography with centrifugal partition chromatography (CPC) was used for the fractionation of partially depolymerized fucans (polysulphated polysaccharides). The ion-exchanger was Amberlite LA2, a high-molecular-mass liquid secondary amine miscible with most common organic solvents and immiscible with aqueous solutions. Ion-exchange displacement centrifugal partition chromatography was performed with LA2 in methyl isobutyl ketone (MiBK) as the stationary phase, water as the mobile phase, Cl- as the carrier and OH- as the displacer. A complex mixture of partially depolymerized fucans was resolved into adjacent families characterized by their peak molecular mass and polydispersity. The Dubois test (sugar) and the azur A test (SO3-) confirmed the displacement mode of the process, and size-exclusion chromatographic controls confirmed its efficiency.
一种将离子交换置换色谱法与离心分配色谱法（CPC）相结合的新方法被用于部分解聚的岩藻聚糖（多硫酸化多糖）的分级分离。离子交换剂为Amberlite LA2，一种高分子质量的液态仲胺，可与大多数常见有机溶剂混溶，与水溶液不混溶。以LA2在甲基异丁基酮（MiBK）中作为固定相、水作为流动相、Cl-作为载体、OH-作为置换剂进行离子交换置换离心分配色谱法。部分解聚的岩藻聚糖的复杂混合物被分离成以其峰值分子量和多分散性为特征的相邻族。杜波依斯试验（糖）和天青A试验（SO3-）证实了该过程的置换模式，尺寸排阻色谱对照证实了其效率。
