Birebent B, Semana G, Commeurec A, Edan G, Genetet B, Genetet N
G.U.R.I.F.A, U.F.R. des Sciences Medicales, Universite de Rennes 1, France.
J Neurosci Res. 1998 Mar 15;51(6):759-70. doi: 10.1002/(SICI)1097-4547(19980315)51:6<759::AID-JNR9>3.0.CO;2-A.
The cerebrospinal fluid (CSF) represents an important source of T lymphocytes that could be involved in the inflammatory response occurring in the central nervous system in multiple sclerosis (MS). In order to investigate whether the Vbeta gene usage of CSF T lymphocytes is restricted, we analyzed the TCR Vbeta expression in twelve CSF expanded by in vitro culture compared to the paired in vitro-stimulated peripheral blood T lymphocytes. The overexpression of one or two Vbeta genes was demonstrated in ten CSF, but the type of Vbeta over expressed varied from one patient to another. For one patient, the Vbeta repertoire was also investigated by single cell cloning. High frequency of BV6S7-expressing T cell clones was observed in the CSF while no BV6S7 clone was derived from the peripheral blood T lymphocytes suggesting that these cells could be involved in the immunopathological process in the central nervous system (CNS). The cytokine patterns of the T cell clones derived from the CSF- and peripheral blood-T lymphocytes of this patient were determined. The CSF T cell clones produced higher levels of cytokines than the peripheral blood T cell clones. The high frequency of IL-4-producing-T cell clones observed in CSF demonstrate that T cells which could downregulate the inflammatory process are present in the CNS.
脑脊液（CSF）是T淋巴细胞的一个重要来源，这些T淋巴细胞可能参与多发性硬化症（MS）中枢神经系统中发生的炎症反应。为了研究CSF T淋巴细胞的Vβ基因使用是否受限我们分析了12份经体外培养扩增的CSF中TCR Vβ的表达，并与配对的体外刺激外周血T淋巴细胞进行比较。在10份CSF中证实了一种或两种Vβ基因的过表达，但不同患者中过表达的Vβ类型各不相同。对于一名患者，还通过单细胞克隆研究了Vβ库。在CSF中观察到表达BV6S7的T细胞克隆频率很高，而外周血T淋巴细胞未产生BV6S7克隆，这表明这些细胞可能参与中枢神经系统（CNS）的免疫病理过程。测定了该患者CSF和外周血T淋巴细胞来源的T细胞克隆的细胞因子谱。CSF T细胞克隆产生的细胞因子水平高于外周血T细胞克隆。在CSF中观察到产生IL-4的T细胞克隆频率很高，这表明中枢神经系统中存在可下调炎症过程的T细胞。
