叙利亚仓鼠新型细胞色素P450 3A（CYP3A31）的cDNA克隆与表达
cDNA cloning and expression of a novel CYP3A from the Syrian hamster, CYP3A31.
作者信息
Alabouch S, Kurose K, Tohkin M, Bani M H, Fukuhara M, Nagata K, Yamazoe Y
机构信息
Department of Pharmaceutical Sciences, National Institute of Public Health, Tokyo, Japan.
出版信息
Biochim Biophys Acta. 1998 Apr 1;1397(1):9-13. doi: 10.1016/s0167-4781(97)00220-0.
A clone, encoding a cytochrome P450 protein consisting of 501 amino acids, was isolated from a cDNA library constructed from mRNA of Syrian hamster liver. The deduced amino acid sequence of this clone showed a high homology (65 to 81%) with other mammalian CYP3As and hence, this novel isozyme was named CYP3A31. By Northern blotting, using an oligonucleotide specific to CYP3A31, the mRNA for this isozyme was shown to be expressed constitutively in liver and induced by treatment with phenobarbital but repressed by 3-methylcholanthrene or dexamethasone treatments. The increase in mRNA expression by phenobarbital and decrease by dexamethasone corresponded to changes in CYP3A protein as analysed by Western blotting. These indicate that CYP3A31 might constitute one of the major CYP3A isozymes in the hamster.
从叙利亚仓鼠肝脏mRNA构建的cDNA文库中分离出一个克隆，它编码一种由501个氨基酸组成的细胞色素P450蛋白。该克隆推导的氨基酸序列与其他哺乳动物CYP3A具有高度同源性（65%至81%），因此，这种新的同工酶被命名为CYP3A31。通过使用针对CYP3A31的寡核苷酸进行Northern印迹分析，发现该同工酶的mRNA在肝脏中组成性表达，经苯巴比妥处理可诱导表达，但经3-甲基胆蒽或地塞米松处理则受到抑制。苯巴比妥使mRNA表达增加，地塞米松使其表达减少，这与通过Western印迹分析的CYP3A蛋白变化相对应。这些表明CYP3A31可能是仓鼠中主要的CYP3A同工酶之一。
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