Seo H C, Ellingsen S, Fjose A
Department of Molecular Biology, University of Bergen, HIB-Biobuilding, N-5020, Bergen, Norway.
Mech Dev. 1998 Apr;73(1):45-57. doi: 10.1016/s0925-4773(98)00028-8.
The murine homeobox gene Six3 and its Drosophila homologue sine oculis both have regulatory functions in eye development. We report the isolation and characterization of two zebrafish genes, six3 and six6, that are closely related to the murine Six3 gene. Zebrafish six3 may be the structural orthologue, while the six6 gene is more similar with respect to embryonic expression. Transcripts of both zebrafish six genes are first detected in involuting axial mesendoderm and, subsequently, in the overlying anterior neural plate from which the optic vesicles and the forebrain will develop. Direct correspondence between six3/six6 expression boundaries and the optic vesicles indicate essential roles in defining the eye primordia. During later stages only the six6 gene displays similar features of expression in the eyes and rostral brain as reported previously for murine Six3.
小鼠同源异型框基因Six3及其果蝇同源基因无眼均在眼睛发育中具有调控功能。我们报告了两个与小鼠Six3基因密切相关的斑马鱼基因six3和six6的分离与特征。斑马鱼six3可能是结构直系同源基因，而six6基因在胚胎表达方面更为相似。斑马鱼这两个six基因的转录本首先在卷入的轴中胚层中被检测到，随后在覆盖其上的前神经板中被检测到，视泡和前脑将从该前神经板发育而来。six3/six6表达边界与视泡之间的直接对应关系表明它们在定义眼原基中起着重要作用。在后期阶段，只有six6基因在眼睛和吻端脑区表现出与之前报道的小鼠Six3相似的表达特征。
