Taketani Y, Miyamoto K, Chikamori M, Tanaka K, Yamamoto H, Tatsumi S, Morita K, Takeda E
Department of Clinical Nutrition, School of Medicine, University of Tokushima, Japan.
Biochim Biophys Acta. 1998 Mar 13;1396(3):267-72. doi: 10.1016/s0167-4781(97)00231-5.
To elucidate the expression and regulation of the human type I Na+/phosphate transporter gene (NPT-1), the 5' flanking region of the NPT-1 gene was cloned, and its nucleotide sequence and function were determined. A genomic clone that contained approximately 14.0 kb of the 5'-flanking region of the NPT-1 gene was isolated. A single transcription start site was located 104 base pairs (bp) upstream of the 3' end of exon 1. In addition to the sequence of the 5'-flanking region contained a sequence weakly homologous to a TATA box at position -41 to -36 and many transcriptional regulatory elements. Transient expression revealed that a 45-bp region of proximal to exon 1, which contained TATA-like sequence, was sufficient for promoting luciferase expression in OK-cells derived from opossum kidney proximal tubule.
为阐明人类I型钠/磷酸盐转运体基因（NPT-1）的表达及调控机制，克隆了NPT-1基因的5'侧翼区，并测定了其核苷酸序列和功能。分离出一个包含NPT-1基因约14.0 kb 5'侧翼区的基因组克隆。在第1外显子3'端上游104个碱基对（bp）处定位到一个单一转录起始位点。除了5'侧翼区序列外，在-41至-36位还含有一个与TATA盒弱同源的序列以及许多转录调控元件。瞬时表达显示，第1外显子近端一个含有TATA样序列的45 bp区域足以促进来自负鼠肾近端小管的OK细胞中荧光素酶的表达。
