Merriman J A, Nieland M L, Wedmore R J
J Microsc. 1979 Jul;116(2):243-53. doi: 10.1111/j.1365-2818.1979.tb00205.x.
Keratinocytes and melanocytes cultured from guinea-pig epidermis were studied with scanning, transmission and high voltage electron microscopy to characterize the surface and internal morphology. Keratinocytes exhibited contact-inhibition and a range of surface structures consistent with cell-cycle dependent changes. Stereoscopic analysis of high voltage electron micrographs indicated regular oval nuclei with nucleoli at different depths, while thin sections revealed local channels in the nuclei. Secondary cultures differed from primary cultures in the disorder of the microfilaments, in the failure to form desmosomes, and in the failure of melanocytes to persist in culture. The beaded surface of melanocytes was indicative of underlying melanosomes that were seen in high voltage micrographs. Melanocytes were rounded with moderate ruffles or were dendritic with ruffles on the termini. These findings are discussed in relation to the observational techniques and in relation to modes of locomotion of and pigment transfer to epidermal cells.
利用扫描电子显微镜、透射电子显微镜和高压电子显微镜对从豚鼠表皮培养的角质形成细胞和黑素细胞进行了研究，以表征其表面和内部形态。角质形成细胞表现出接触抑制以及一系列与细胞周期依赖性变化一致的表面结构。对高压电子显微照片的立体分析表明，细胞核呈规则的椭圆形，核仁位于不同深度，而超薄切片显示细胞核内有局部通道。继代培养与原代培养的不同之处在于微丝紊乱、未能形成桥粒以及黑素细胞在培养中不能持续存在。黑素细胞的串珠状表面表明在高压显微照片中可见其下方的黑素体。黑素细胞呈圆形，有适度的褶皱，或呈树突状，末端有褶皱。结合观察技术以及表皮细胞的运动方式和色素转移方式对这些发现进行了讨论。
