盘基网柄菌延伸因子-1β编码cDNA的克隆与序列分析

Cloning and sequence analysis of the cDNA encoding the elongation factor-1 beta of Dictyostelium discoideum.

作者信息

Chae S, Maeda Y

机构信息

Biological Institute, Graduate School of Science, Tohoku University, Sendai, Japan.

出版信息

Biochim Biophys Acta. 1998 Mar 3;1383(1):1-3. doi: 10.1016/s0167-4838(97)00220-3.

DOI:10.1016/s0167-4838(97)00220-3

PMID:9546041

Abstract

A cDNA encoding elongation factor-1 beta (DdEF-1 beta) for protein synthesis was isolated from Dictyostlium discoideum cells by the differential display (DD) method. The DdEF-1 beta cDNA sequence with a length of 733 bp encodes a protein (M(r) 24.1 kDa) consisting of 214 amino acids. Comparison of the predicted amino acid sequence revealed relatively high homology to that of DdEF-1 beta from silkworm (59%), human (57%) and Xenopus (55%). Northern analysis showed that the DdEF-1 beta mRNA is maximally expressed during the vegetative growth phase, followed by marked decrease in response to cell differentiation induced by starvation.

摘要

通过差异显示（DD）方法，从盘基网柄菌细胞中分离出一个编码用于蛋白质合成的延伸因子-1β（DdEF-1β）的cDNA。长度为733 bp的DdEF-1β cDNA序列编码一个由214个氨基酸组成的蛋白质（分子量24.1 kDa）。预测的氨基酸序列比较显示，其与家蚕的DdEF-1β（59%）、人类的DdEF-1β（57%）和非洲爪蟾的DdEF-1β（55%）具有较高的同源性。Northern分析表明，DdEF-1β mRNA在营养生长阶段表达最高，随后在饥饿诱导的细胞分化反应中显著下降。