Takahashi K
Department of Hygiene and Preventive Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1998 Jan;73(1):61-71.
The present study was performed to elucidate the effects of physical fatigue on immune parameters of peripheral blood and splenic T cells in rats. Male Wistar rats aged 20 to 45 weeks were used for the experiment. Swimming was employed as a physical fatigue load. The rats in the single-load group with a weight equal to 3% of the body weight of each rat connected to the tails were exhausted by two hours of swimming in one day. The rats in the consecutive-load group were exhausted by three hours of swimming for 7 consecutive days with a weight equal to 5% of the body weight of each rat. Absolute numbers of total white blood cells (WBCs) and peripheral blood lymphocytes decreased significantly just after swimming and returned to the baseline one day after swimming in both the single- and consecutive-load groups. However, in the consecutive-load group WBCs and lymphocytes decreased 7 days after swimming. Concentrations of plasma corticosterone increased significantly just after swimming, and returned to the baseline one day after swimming in both groups. Percentages of peripheral blood CD5+ cells, CD4+ cells and CD8+ cells in the single-load group increased significantly just after swimming. The percentage of peripheral blood CD5+ cells and CD4+ cells increased significantly one day after swimming in the consecutive load group. Percentages of splenic CD5+ cells and CD4+ cells increased significantly in the single-load group. However, only that of CD4+ cells increased significantly just after swimming in the consecutive-load group. A single load affected the proportions of CD5+ cells, CD4+ cells and CD8+ cells just after the load but the consecutive load affected CD5+ cells and CD4+ cells and the effect persisted until the next day. These results indicate that the effect of fatigue on immune parameters depends on the level of fatigue, and that measurement of subsets of peripheral blood T cells is useful for evaluating various levels of fatigue.
本研究旨在阐明身体疲劳对大鼠外周血免疫参数及脾T细胞的影响。选用20至45周龄的雄性Wistar大鼠进行实验。采用游泳作为身体疲劳负荷。单负荷组大鼠尾巴连接相当于其体重3%的重物，一天游泳两小时直至精疲力竭。连续负荷组大鼠尾巴连接相当于其体重5%的重物，连续7天每天游泳3小时直至精疲力竭。单负荷组和连续负荷组大鼠游泳刚结束后，外周血白细胞（WBC）总数和淋巴细胞绝对数量均显著下降，游泳一天后恢复至基线水平。然而，连续负荷组大鼠游泳7天后WBC和淋巴细胞数量下降。两组大鼠游泳刚结束后血浆皮质酮浓度均显著升高，游泳一天后恢复至基线水平。单负荷组大鼠游泳刚结束后外周血CD5⁺细胞、CD4⁺细胞和CD8⁺细胞百分比显著升高。连续负荷组大鼠游泳一天后外周血CD5⁺细胞和CD4⁺细胞百分比显著升高。单负荷组大鼠脾CD5⁺细胞和CD4⁺细胞百分比显著升高。然而，连续负荷组大鼠仅在游泳刚结束后CD4⁺细胞百分比显著升高。单次负荷在负荷后即刻影响CD5⁺细胞、CD4⁺细胞和CD8⁺细胞比例，但连续负荷影响CD5⁺细胞和CD4⁺细胞比例，且这种影响持续至第二天。这些结果表明，疲劳对免疫参数的影响取决于疲劳程度，且外周血T细胞亚群的检测有助于评估不同程度的疲劳。
