捕获HIV-1 gp41的早期融合活性构象。
Capture of an early fusion-active conformation of HIV-1 gp41.
作者信息
Furuta R A, Wild C T, Weng Y, Weiss C D
机构信息
Office of Vaccines, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892-4555, USA.
出版信息
Nat Struct Biol. 1998 Apr;5(4):276-9. doi: 10.1038/nsb0498-276.
PMID:9546217
Abstract
Using an inhibitory synthetic peptide (DP-178) from HIV-1 gp41, we have trapped HIV-1 envelope glycoprotein (Env) undergoing conformational changes during virus entry. Our data show that DP-178 binds gp41 and inhibits Env-mediated membrane fusion after gp120 interacts with cellular receptors, indicating that conformational changes involving the coiled coil domain of gp41 are required for entry. Capture of this fusion-active conformation of Env provides insights into the early events leading to Env-mediated membrane fusion.
摘要
利用来自HIV-1 gp41的抑制性合成肽（DP-178），我们捕获到了在病毒进入过程中发生构象变化的HIV-1包膜糖蛋白（Env）。我们的数据表明，DP-178在gp120与细胞受体相互作用后结合gp41并抑制Env介导的膜融合，这表明涉及gp41卷曲螺旋结构域的构象变化是病毒进入所必需的。捕获Env的这种融合活性构象为深入了解导致Env介导膜融合的早期事件提供了线索。
相似文献
1
Capture of an early fusion-active conformation of HIV-1 gp41.捕获HIV-1 gp41的早期融合活性构象。
Nat Struct Biol. 1998 Apr;5(4):276-9. doi: 10.1038/nsb0498-276.
2
Selection of gp41-mediated HIV-1 cell entry inhibitors from biased combinatorial libraries of non-natural binding elements.从非天然结合元件的偏向组合文库中筛选gp41介导的HIV-1细胞进入抑制剂。
Nat Struct Biol. 1999 Oct;6(10):953-60. doi: 10.1038/13324.
3
Sensitivity of HIV-1 to entry inhibitors correlates with envelope/coreceptor affinity, receptor density, and fusion kinetics.人类免疫缺陷病毒1型（HIV-1）对进入抑制剂的敏感性与包膜/共受体亲和力、受体密度及融合动力学相关。
Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16249-54. doi: 10.1073/pnas.252469399. Epub 2002 Nov 20.
4
Mode of action of an antiviral peptide from HIV-1. Inhibition at a post-lipid mixing stage.一种来自HIV-1的抗病毒肽的作用模式。在脂质混合后阶段的抑制作用。
J Biol Chem. 2001 Jan 12;276(2):1391-7. doi: 10.1074/jbc.M004113200.
5
Role of the ectodomain of the gp41 transmembrane envelope protein of human immunodeficiency virus type 1 in late steps of the membrane fusion process.人类免疫缺陷病毒1型gp41跨膜包膜蛋白胞外结构域在膜融合过程后期步骤中的作用。
J Virol. 2004 Jan;78(2):811-20. doi: 10.1128/jvi.78.2.811-820.2004.
6
HIV-1 gp41 Residues Modulate CD4-Induced Conformational Changes in the Envelope Glycoprotein and Evolution of a Relaxed Conformation of gp120.HIV-1 gp41 残基调节包膜糖蛋白中 CD4 诱导的构象变化和 gp120 构象的弛豫演化。
J Virol. 2018 Jul 31;92(16). doi: 10.1128/JVI.00583-18. Print 2018 Aug 15.
7
Role of the fusion peptide and membrane-proximal domain in HIV-1 envelope glycoprotein-mediated membrane fusion.融合肽和膜近端结构域在HIV-1包膜糖蛋白介导的膜融合中的作用。
Biochemistry. 2003 Dec 9;42(48):14150-8. doi: 10.1021/bi035154g.
8
Covalent stabilization of coiled coils of the HIV gp41 N region yields extremely potent and broad inhibitors of viral infection.HIV gp41 N区域卷曲螺旋的共价稳定作用产生了极其强效且广谱的病毒感染抑制剂。
Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12903-8. doi: 10.1073/pnas.0502449102. Epub 2005 Aug 29.
9
A synthetic all D-amino acid peptide corresponding to the N-terminal sequence of HIV-1 gp41 recognizes the wild-type fusion peptide in the membrane and inhibits HIV-1 envelope glycoprotein-mediated cell fusion.一种与HIV-1 gp41 N端序列相对应的合成全D-氨基酸肽可识别膜中的野生型融合肽，并抑制HIV-1包膜糖蛋白介导的细胞融合。
Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7287-92. doi: 10.1073/pnas.95.13.7287.
10
Design and properties of N(CCG)-gp41, a chimeric gp41 molecule with nanomolar HIV fusion inhibitory activity.具有纳摩尔级HIV融合抑制活性的嵌合gp41分子N(CCG)-gp41的设计与特性
J Biol Chem. 2001 Aug 3;276(31):29485-9. doi: 10.1074/jbc.C100317200. Epub 2001 Jun 19.
引用本文的文献
1
Incompletely closed HIV-1 envelope glycoproteins resist broadly neutralizing antibodies while mediating efficient HIV-1 entry.未完全封闭的HIV-1包膜糖蛋白可抵御广泛中和抗体，同时介导高效的HIV-1进入。
Npj Viruses. 2025 Jan 15;3(1):3. doi: 10.1038/s44298-024-00082-w.
2
Capture of fusion-intermediate conformations of SARS-CoV-2 spike requires receptor binding and cleavage at either the S1/S2 or S2' site.捕获严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突蛋白的融合中间构象需要受体结合以及在S1/S2或S2'位点的切割。
PLoS Pathog. 2025 Apr 8;21(4):e1012808. doi: 10.1371/journal.ppat.1012808. eCollection 2025 Apr.
3
The membrane-proximal external region of human immunodeficiency virus (HIV-1) envelope glycoprotein trimers in A18-lipid nanodiscs.A18脂质纳米盘中人类免疫缺陷病毒（HIV-1）包膜糖蛋白三聚体的膜近端外部区域。
Commun Biol. 2025 Mar 15;8(1):442. doi: 10.1038/s42003-025-07852-z.
4
Inducible cell lines producing replication-defective human immunodeficiency virus particles containing envelope glycoproteins stabilized in a pretriggered conformation.可诱导细胞系产生含有以预触发构象稳定化的包膜糖蛋白的复制缺陷型人类免疫缺陷病毒颗粒。
J Virol. 2024 Dec 17;98(12):e0172024. doi: 10.1128/jvi.01720-24. Epub 2024 Nov 7.
5
Conformational flexibility of HIV-1 envelope glycoproteins modulates transmitted/founder sensitivity to broadly neutralizing antibodies.HIV-1 包膜糖蛋白的构象灵活性调节了传播/原始病毒对广泛中和抗体的敏感性。
Nat Commun. 2024 Aug 26;15(1):7334. doi: 10.1038/s41467-024-51656-4.
6
HIResist: a database of HIV-1 resistance to broadly neutralizing antibodies.HIResist：一个 HIV-1 对广泛中和抗体的耐药性数据库。
Bioinformatics. 2024 Mar 4;40(3). doi: 10.1093/bioinformatics/btae103.
7
Inhibition of human immunodeficiency virus (HIV-1) infectivity by expression of poorly or broadly neutralizing antibodies against Env in virus-producing cells.在产生病毒的细胞中表达针对 Env 的中和活性弱或广谱中和抗体抑制人类免疫缺陷病毒（HIV-1）感染。
J Virol. 2024 Feb 20;98(2):e0159423. doi: 10.1128/jvi.01594-23. Epub 2024 Jan 30.
8
V3 tip determinants of susceptibility to inhibition by CD4-mimetic compounds in natural clade A human immunodeficiency virus (HIV-1) envelope glycoproteins.V3 尖端决定簇对天然 A 群人类免疫缺陷病毒（HIV-1）包膜糖蛋白中 CD4 模拟化合物抑制敏感性的影响。
J Virol. 2023 Nov 30;97(11):e0117123. doi: 10.1128/jvi.01171-23. Epub 2023 Oct 27.
9
Conformational flexibility of HIV-1 envelope glycoproteins modulates transmitted / founder sensitivity to broadly neutralizing antibodies.HIV-1包膜糖蛋白的构象灵活性调节了传播/奠基者病毒对广泛中和抗体的敏感性。
bioRxiv. 2023 Dec 5:2023.09.13.557082. doi: 10.1101/2023.09.13.557082.
10
Alterations in gp120 glycans or the gp41 fusion peptide-proximal region modulate the stability of the human immunodeficiency virus (HIV-1) envelope glycoprotein pretriggered conformation.糖基化或 gp41 融合肽近端区的改变调节人类免疫缺陷病毒 (HIV-1) 包膜糖蛋白预触发构象的稳定性。
J Virol. 2023 Sep 28;97(9):e0059223. doi: 10.1128/jvi.00592-23. Epub 2023 Sep 11.
Zotero 插件