Furuta R A, Wild C T, Weng Y, Weiss C D
Office of Vaccines, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892-4555, USA.
Nat Struct Biol. 1998 Apr;5(4):276-9. doi: 10.1038/nsb0498-276.
Using an inhibitory synthetic peptide (DP-178) from HIV-1 gp41, we have trapped HIV-1 envelope glycoprotein (Env) undergoing conformational changes during virus entry. Our data show that DP-178 binds gp41 and inhibits Env-mediated membrane fusion after gp120 interacts with cellular receptors, indicating that conformational changes involving the coiled coil domain of gp41 are required for entry. Capture of this fusion-active conformation of Env provides insights into the early events leading to Env-mediated membrane fusion.
利用来自HIV-1 gp41的抑制性合成肽（DP-178），我们捕获到了在病毒进入过程中发生构象变化的HIV-1包膜糖蛋白（Env）。我们的数据表明，DP-178在gp120与细胞受体相互作用后结合gp41并抑制Env介导的膜融合，这表明涉及gp41卷曲螺旋结构域的构象变化是病毒进入所必需的。捕获Env的这种融合活性构象为深入了解导致Env介导膜融合的早期事件提供了线索。
