Dirmeier R, Keller M, Frey G, Huber H, Stetter K O
Lehrstuhl für Mikrobiologie, Regensburg, Germany.
Eur J Biochem. 1998 Mar 15;252(3):486-91. doi: 10.1046/j.1432-1327.1998.2520486.x.
The chemolithoautotrophic archaeon Pyrodictium abyssi isolate TAG 11 gains energy by reducing sulfur with H2 to H2S. From this hyperthermophile, a sulfur-reducing complex catalyzing this reaction was purified 13.5-fold. The native complex exhibited a brownish-yellow colour and showed an apparent molecular mass of 520 kDa. SDS/PAGE revealed the presence of nine different major polypeptides with apparent molecular masses of 82, 72, 65, 50, 47, 42, 40, 30 and 24 kDa. The native complex contained 50-55 mol acid-labile sulfur, 50-55 mol iron, 1.6 mol nickel, 1.2 mol copper, 2.8 mol cytochrome b and 0.3 mol cytochrome c (all per mol native complex). The temperature optimum of the H2:sulfur oxidoreductase complex was 100 degrees C, which is consistent with the physiological growth optimum of the native organism. The complex is extremely heat stable. During 5 h incubation at 100 degrees C, no decrease in H2S-forming activity could be observed.
化能无机自养古菌深渊嗜热栖热菌TAG 11菌株通过利用氢气将硫还原为硫化氢来获取能量。从这种嗜热菌中纯化出了催化该反应的硫还原复合物，纯化倍数为13.5倍。天然复合物呈现出棕黄色，表观分子量为520 kDa。SDS/PAGE分析显示存在9种不同的主要多肽，其表观分子量分别为82、72、65、50、47、42、40、30和24 kDa。天然复合物每摩尔含有50 - 55摩尔酸不稳定硫、50 - 55摩尔铁、1.6摩尔镍、1.2摩尔铜、2.8摩尔细胞色素b和0.3摩尔细胞色素c（均相对于每摩尔天然复合物）。氢气：硫氧化还原酶复合物的最适温度为100℃，这与天然生物体的生理生长最适温度一致。该复合物具有极高的热稳定性。在100℃孵育5小时期间，未观察到形成硫化氢活性的降低。
