Brühl P, Kerschbaum A, Eibl M M, Mannhalter J W
Department of Immunological Research, Immuno AG, Vienna, Austria.
AIDS Res Hum Retroviruses. 1998 Mar 20;14(5):401-7. doi: 10.1089/aid.1998.14.401.
Induction of mucosal as well as systemic immunity to HIV-1 is considered to have high priority in current concepts of future AIDS vaccines. Here we show that the desired immune responses can be elicited by an experimental prime-boost regimen consisting of mucosal (intragastric) application of a recombinant vaccinia virus carrying the HIV-1 env gene (vSC25), followed by parenteral (intradermal) immunization with the recombinant HIV-1 glycoprotein 160 (rgp160). Following intragastric immunization of mice with vSC25 in combination with the mucosal adjuvant cholera toxin (CT), HIV-1 env-specific IgA was secreted by B cells of Peyer's patches and the lamina propria. Moreover, mucosal (intragastric and intranasal) application of vSC25 (both in presence or absence of CT) induced a long-lasting, HIV-1 env-specific systemic cytotoxic T cell response. Subsequent intradermal boosters with rgp160 led to HIV-1-specific T cell memory and serum antibodies.
在当前未来艾滋病疫苗的概念中，诱导针对HIV-1的黏膜免疫和全身免疫被视为具有高度优先性。在此我们表明，通过一种实验性的初免-加强方案可以引发所需的免疫反应，该方案包括经黏膜（胃内）应用携带HIV-1 env基因的重组痘苗病毒（vSC25），随后用重组HIV-1糖蛋白160（rgp160）进行肠外（皮内）免疫。在用vSC25联合黏膜佐剂霍乱毒素（CT）对小鼠进行胃内免疫后，派尔集合淋巴结和固有层的B细胞分泌了HIV-1 env特异性IgA。此外，vSC25的黏膜（胃内和鼻内）应用（无论有无CT）诱导了持久的、HIV-1 env特异性全身细胞毒性T细胞反应。随后用rgp160进行皮内加强免疫导致了HIV-1特异性T细胞记忆和血清抗体的产生。
