通过寡核苷酸指纹图谱进行比较基因表达谱分析。
Comparative gene expression profiling by oligonucleotide fingerprinting.
作者信息
Meier-Ewert S, Lange J, Gerst H, Herwig R, Schmitt A, Freund J, Elge T, Mott R, Herrmann B, Lehrach H
机构信息
Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, 14195 Berlin, Germany.
出版信息
Nucleic Acids Res. 1998 May 1;26(9):2216-23. doi: 10.1093/nar/26.9.2216.
The use of hybridisation of synthetic oligonucleotides to cDNAs under high stringency to characterise gene sequences has been demonstrated by a number of groups. We have used two cDNA libraries of 9 and 12 day mouse embryos (24 133 and 34 783 clones respectively) in a pilot study to characterise expressed genes by hybridisation with 110 hybridisation probes. We have identified 33 369 clusters of cDNA clones, that ranged in representation from 1 to 487 copies (0.7%). 737 were assigned to known rodent genes, and a further 13 845 showed significant homologies. A total of 404 clusters were identified as significantly differentially represented (P < 0.01) between the two cDNA libraries. This study demonstrates the utility of the fingerprinting approach for the generation of comparative gene expression profiles through the analysis of cDNAs derived from different biological materials.
许多研究小组已证明，在高严格度条件下，通过合成寡核苷酸与互补DNA（cDNA）杂交来鉴定基因序列是可行的。在一项初步研究中，我们使用了两个分别来自9日龄和12日龄小鼠胚胎的cDNA文库（分别有24133个和34783个克隆），通过与110个杂交探针杂交来鉴定表达的基因。我们鉴定出33369个cDNA克隆簇，其代表数量从1到487个拷贝不等（占0.7%）。737个被归为已知的啮齿动物基因，另有13845个显示出显著的同源性。总共鉴定出404个克隆簇在两个cDNA文库之间存在显著差异表达（P < 0.01）。这项研究证明了指纹图谱方法通过分析来自不同生物材料的cDNA来生成比较基因表达谱的实用性。
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