给马投喂裹巧克力花生后尿液中可可碱和咖啡因的检测与测定。

Detection and determination of theobromine and caffeine in urine after administration of chocolate-coated peanuts to horses.

作者信息

Dyke T M, Sams R A

机构信息

Analytical Toxicology Laboratory, College of Veterinary Medicine, Ohio State University, Columbus 43210-1089, USA.

出版信息

J Anal Toxicol. 1998 Mar-Apr;22(2):112-6. doi: 10.1093/jat/22.2.112.

DOI:10.1093/jat/22.2.112

PMID:9547407

Abstract

The objective of this study was to determine the urinary excretion of methylxanthines in horses following ingestion of chocolate over eight days. The study was performed in response to gas chromatography-mass spectrometry (GC-MS) confirmation of the presence of caffeine in a positive urine test in a racehorse. The trainer of the horse alleged that he often administered chocolate-coated peanuts as treats to his horses, and he believed that the ingestion of chocolate was responsible for the positive urine test. The urinary excretion of theobromine and caffeine after the ingestion of chocolate-coated peanuts was investigated in three horses. Enzyme-linked immunoassay (ELISA), high-performance liquid chromatography (HPLC), and GC-MS assays were performed on all urine specimens. Theobromine (HPLC) was detected for 72 h and caffeine (GC-MS) for 48 h after chronic ingestion of chocolate-coated peanuts. Methylxanthines were detected by ELISA for 120 h after administration of chocolate.

摘要

本研究的目的是确定马匹在连续八天摄入巧克力后甲基黄嘌呤的尿排泄情况。该研究是在通过气相色谱-质谱联用仪（GC-MS）确认一匹赛马的尿液检测呈阳性且含有咖啡因后进行的。这匹马的训练师称，他经常给马喂裹巧克力的花生作为零食，并且他认为摄入巧克力是导致尿液检测呈阳性的原因。对三匹马摄入裹巧克力的花生后的可可碱和咖啡因尿排泄情况进行了研究。对所有尿液样本都进行了酶联免疫吸附测定（ELISA）、高效液相色谱法（HPLC）和GC-MS检测。长期摄入裹巧克力的花生后，可可碱（通过HPLC检测）在72小时内可被检测到，咖啡因（通过GC-MS检测）在48小时内可被检测到。在给予巧克力后，ELISA在120小时内都能检测到甲基黄嘌呤。