癌细胞中的胞质12(S)-羟基-5,8,10,14-二十碳四烯酸结合位点

Cytosolic 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid binding sites in carcinoma cells.

作者信息

Herbertsson H, Hammarström S

机构信息

Department of Cell Biology, Linköping University, Sweden.

出版信息

Adv Exp Med Biol. 1997;400A:287-93. doi: 10.1007/978-1-4615-5325-0_41.

DOI:10.1007/978-1-4615-5325-0_41

PMID:9547570

Abstract

12(S)-HETE stimulates gene and cell surface expression of the integrin GPIIb/IIIa in carcinoma cells. The cells have high affinity binding sites for 12(S)-HETE. Analyses of the subcellular distribution and size of these sites showed that cytosol was the predominant location and that the apparent molecular weight was close to 670,000. Besides cytosol, mitochondria, nuclei, and plasma membranes also contained 12(S)-HETE binding sites. The mainly cytosolic location of the binding sites is different from that of other eicosanoid receptors which are G-protein coupled plasma membrane proteins of the rhodopsin gene family.

摘要

12(S)-羟基二十碳四烯酸（12(S)-HETE）刺激癌细胞中整合素GPIIb/IIIa的基因和细胞表面表达。这些细胞对12(S)-HETE具有高亲和力结合位点。对这些位点的亚细胞分布和大小分析表明，胞质溶胶是主要位置，其表观分子量接近670,000。除了胞质溶胶外，线粒体、细胞核和质膜也含有12(S)-HETE结合位点。结合位点主要位于胞质溶胶，这与其他类花生酸受体不同，后者是视紫红质基因家族的G蛋白偶联质膜蛋白。