Maurer J K, Parker R D, Carr G J
Procter & Gamble Co., Miami Valley Laboratories, Cincinnati, Ohio 45253-8707, USA.
Toxicol Pathol. 1998 Mar-Apr;26(2):226-33. doi: 10.1177/019262339802600206.
We believe the development and validation of in vitro alternatives to eliminate the need to use animals in ocular irritation testing must be based on a thorough understanding of the mechanisms of ocular irritation. We have recently undertaken the task of developing such an understanding for a panel of surfactants. The purpose of this study was to expand our current panel of surfactants for which the microscopic changes occurring over time have been characterized. Macroscopic and microscopic findings regarding the ocular irritation of 6 surfactants of relatively unknown irritancy were compared to those of 6 surfactants of known irritancy. The right eye of each rat was treated by placing 10 microliters of a surfactant directly on the cornea. Untreated left eyes served as the controls. At 3 hr and on days 1, 3, and 35, eyes and eyelids were collected for microscopic examination. Collectively, the macroscopic and microscopic findings revealed 3 surfactants to be similar to the mildly irritating surfactants previously studied, and 3 surfactants to be similar to the moderately irritating surfactant previously studied. Information such as this will be important to develop mechanistically based in vitro alternatives to replace the use of animals for ocular irritation testing.
我们认为，开发和验证用于消除眼刺激试验中使用动物需求的体外替代方法，必须基于对眼刺激机制的透彻理解。我们最近承担了一项任务，即对一组表面活性剂形成这样的理解。本研究的目的是扩大我们目前的表面活性剂组，对于该组表面活性剂，已对随时间发生的微观变化进行了表征。将6种刺激性相对未知的表面活性剂与6种已知刺激性的表面活性剂的眼刺激宏观和微观结果进行了比较。通过将10微升表面活性剂直接滴在每只大鼠的角膜上对右眼进行处理。未处理的左眼作为对照。在3小时以及第1、3和35天，收集眼睛和眼睑进行显微镜检查。总体而言，宏观和微观结果显示，3种表面活性剂与先前研究的轻度刺激性表面活性剂相似，3种表面活性剂与先前研究的中度刺激性表面活性剂相似。此类信息对于开发基于机制的体外替代方法以取代用于眼刺激试验的动物使用至关重要。
