• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

fa/fa(祖克)大鼠胰岛的超微结构和分泌异质性。

Ultrastructural and secretory heterogeneity of fa/fa (Zucker) rat islets.

作者信息

Chan C B, Wright G M, Wadowska D W, MacPhail R M, Ireland W P, Sulston K W

机构信息

Department of Anatomy and Physiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.

出版信息

Mol Cell Endocrinol. 1998 Jan 15;136(2):119-29. doi: 10.1016/s0303-7207(97)00220-7.

DOI:10.1016/s0303-7207(97)00220-7
PMID:9548215
Abstract

Many previous studies of obese rodents documented biochemical changes in pancreatic islets that contribute to hyperinsulinemia in vivo. Those studies used heterogeneous populations of islets, although the size of islets from obese rats ranges from < 100 to > 500 microm. Here, functional and morphological changes in size-sorted (< 125 and > 250 microm diameter) islets from obese Zucker (fa/fa) rats were correlated. Ultrastructural examination revealed that > 250 microm cultured islets had an increased number of immature secretory granules in the beta cells. The number of degranulated beta cells in > 250 and < 125 microm cultured islets from fa/fa rats was higher than in lean rat islets (33 vs 25%). The glucose EC50 values for cultured islets were 4.64 +/- 0.43, 7.9 +/- 0.70 and 7.29 +/- 1.64 mmol.l(-1) for > 250 microm, < 125 microm, and lean groups, respectively. Inhibition of insulin secretion by 10 mmol.l(-1) mannoheptulose was reduced by 50% in > 250 microm islets compared with small islets. Studies of individual beta cells by reverse hemolytic plaque assay revealed 3-fold more cells from > 250 microm islets were stimulated by 1.4 mmol.l(-1) glucose than cells from < 125 microm islets. We conclude that functional defects in mixed size populations of islets from fa/fa rats are mainly due to alterations in the large islets, whereas smaller islets have relatively normal function. Exposure to high glucose exacerbates morphological and functional differences of large islets, which could have important implications in the transition to noninsulin-dependent diabetes when beta cell insulin production is unable to compensate for hyperglycemia.

摘要

此前许多针对肥胖啮齿动物的研究记录了胰岛中的生化变化,这些变化在体内导致了高胰岛素血症。那些研究使用的是异质性胰岛群体,尽管肥胖大鼠的胰岛大小范围在小于100微米至大于500微米之间。在此,对肥胖Zucker(fa/fa)大鼠按大小分选(直径小于125微米和大于250微米)的胰岛的功能和形态变化进行了关联研究。超微结构检查显示,直径大于250微米的培养胰岛中β细胞内未成熟分泌颗粒数量增加。来自fa/fa大鼠的直径大于250微米和小于125微米的培养胰岛中脱颗粒β细胞的数量高于瘦大鼠胰岛(分别为33%和25%)。培养胰岛的葡萄糖EC50值,直径大于250微米、小于125微米和瘦大鼠组分别为4.64±0.43、7.9±0.70和7.29±1.64毫摩尔/升。与小胰岛相比,10毫摩尔/升甘露庚酮对直径大于250微米胰岛胰岛素分泌的抑制作用降低了50%。通过反向溶血空斑试验对单个β细胞的研究显示,1.4毫摩尔/升葡萄糖刺激下,直径大于250微米胰岛中的细胞受到刺激的数量比直径小于125微米胰岛中的细胞多3倍。我们得出结论,fa/fa大鼠混合大小胰岛群体中的功能缺陷主要归因于大胰岛的改变,而较小的胰岛功能相对正常。暴露于高葡萄糖会加剧大胰岛的形态和功能差异,这在β细胞胰岛素生成无法补偿高血糖向非胰岛素依赖型糖尿病转变过程中可能具有重要意义。

相似文献

1
Ultrastructural and secretory heterogeneity of fa/fa (Zucker) rat islets.fa/fa(祖克)大鼠胰岛的超微结构和分泌异质性。
Mol Cell Endocrinol. 1998 Jan 15;136(2):119-29. doi: 10.1016/s0303-7207(97)00220-7.
2
Effect of adrenalectomy on the development of a pancreatic islet lesion in fa/fa rats.肾上腺切除术对fa/fa大鼠胰岛病变发展的影响。
Diabetologia. 1996 Feb;39(2):190-8. doi: 10.1007/BF00403962.
3
Glucose refractoriness of beta-cells from fed fa/fa rats is ameliorated by nonesterified fatty acids.非酯化脂肪酸可改善喂食后的fa/fa大鼠β细胞的葡萄糖抵抗性。
Can J Physiol Pharmacol. 1999 Dec;77(12):934-42.
4
Glucose-inducible hypertrophy and suppression of anion efflux in rat beta cells.
J Endocrinol. 2002 Apr;173(1):45-52. doi: 10.1677/joe.0.1730045.
5
Beta-cell hypertrophy in fa/fa rats is associated with basal glucose hypersensitivity and reduced SNARE protein expression.肥胖(fa/fa)大鼠的β细胞肥大与基础葡萄糖超敏反应及SNARE蛋白表达降低有关。
Diabetes. 1999 May;48(5):997-1005. doi: 10.2337/diabetes.48.5.997.
6
Modulation by glucose of insulin secretion and glucose phosphorylating activity in cultured pancreatic islets from obese (fa/fa) Zucker rats.葡萄糖对肥胖型(fa/fa) Zucker大鼠培养胰岛中胰岛素分泌及葡萄糖磷酸化活性的调节作用
Int J Obes Relat Metab Disord. 1996 Feb;20(2):175-84.
7
KATP channel-dependent and -independent pathways of insulin secretion in isolated islets from fa/fa Zucker rats.肥胖 Zucker 大鼠分离胰岛中胰岛素分泌的 KATP 通道依赖性和非依赖性途径
Biochem Cell Biol. 1996;74(3):403-10. doi: 10.1139/o96-043.
8
Glucokinase activity in isolated islets from obese fa/fa Zucker rats.肥胖型fa/fa Zucker大鼠分离胰岛中的葡萄糖激酶活性
Biochem J. 1993 Nov 1;295 ( Pt 3)(Pt 3):673-7. doi: 10.1042/bj2950673.
9
Evidence for defective glucose sensing by islets of fa/fa obese Zucker rats.fa/fa肥胖型 Zucker 大鼠胰岛葡萄糖感应缺陷的证据。
Can J Physiol Pharmacol. 1993 Jan;71(1):34-9. doi: 10.1139/y93-005.
10
Augmented insulinotropic action of arachidonic acid through the lipoxygenase pathway in the obese Zucker rat.肥胖型 Zucker 大鼠中花生四烯酸通过脂氧合酶途径增强促胰岛素作用。
Obes Res. 2000 Sep;8(6):475-80. doi: 10.1038/oby.2000.59.

引用本文的文献

1
Development of complex pedagogical competencies and reflexivity in clinical teachers via distance learning: a mixed methods study.通过远程学习发展临床教师的复杂教学能力和反思能力:混合方法研究。
Med Educ Online. 2023 Dec;28(1):2265163. doi: 10.1080/10872981.2023.2265163. Epub 2023 Oct 11.
2
Tumor-like features of gene expression and metabolic profiles in enlarged pancreatic islets are associated with impaired incretin-induced insulin secretion in obese diabetes: A study of Zucker fatty diabetes mellitus rat.肥胖型糖尿病 Zucker 脂肪糖尿病大鼠胰岛增大时基因表达和代谢谱的肿瘤样特征与肠降血糖素诱导胰岛素分泌受损有关。
J Diabetes Investig. 2020 Nov;11(6):1434-1447. doi: 10.1111/jdi.13272. Epub 2020 May 29.
3
Low insulin content of large islet population is present in situ and in isolated islets.
大胰岛群体的胰岛素含量低,这种情况既存在于原位,也存在于分离的胰岛中。
Islets. 2011 Jan-Feb;3(1):6-13. doi: 10.4161/isl.3.1.14132.
4
Fetal and neonatal nicotine exposure in Wistar rats causes progressive pancreatic mitochondrial damage and beta cell dysfunction.Wistar大鼠的胎儿和新生儿尼古丁暴露会导致胰腺线粒体进行性损伤和β细胞功能障碍。
PLoS One. 2008;3(10):e3371. doi: 10.1371/journal.pone.0003371. Epub 2008 Oct 8.