Hou W C, Lin Y H
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, R.O.C.
Electrophoresis. 1998 Feb;19(2):212-4. doi: 10.1002/elps.1150190212.
The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57 on a 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.
通过在染色前将凝胶浸入含有10-40 mM过氧化氢、10 mM Tris缓冲液（pH 7.9）的溶液中30分钟，可防止包括台农27、台农34和台农57在内的甘薯品种粗叶提取物中的胰蛋白酶抑制剂在15%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶上的活性染色失败。
